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Literature summary for 3.2.1.97 extracted from

  • Kobata, A.; Takasaki, S.
    endo-beta-Galactosidase and endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae (1978), Methods Enzymol., 50, 560-584.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is useful in the study of the structures of the carbohydrate moieties of various complex carbohydrates Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2.5 mM, 63% inhibition Streptococcus pneumoniae
Mn2+ 2.5 mM, 44% inhibition Streptococcus pneumoniae
p-chloromercuribenzenesulfonate 1 mM, 60% inhibition Streptococcus pneumoniae
Zn2+ 2.5 mM, 66% inhibition Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at a level twice the EDTA concentration completely restores enzyme activity Streptococcus pneumoniae
Mg2+ at a level twice the EDTA concentration completely restores enzyme activity Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.5
-
-
Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycopeptide + H2O release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages Streptococcus pneumoniae Galbeta(1-3)GalNAc + ?
-
?
glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Streptococcus pneumoniae Galbeta(1-3)GalNAc + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about 50% of maximal activity at pH 5.5 and at pH 7.0 Streptococcus pneumoniae