Application | Comment | Organism |
---|---|---|
analysis | the enzyme is useful in the study of the structures of the carbohydrate moieties of various complex carbohydrates | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2.5 mM, 63% inhibition | Streptococcus pneumoniae | |
Mn2+ | 2.5 mM, 44% inhibition | Streptococcus pneumoniae | |
p-chloromercuribenzenesulfonate | 1 mM, 60% inhibition | Streptococcus pneumoniae | |
Zn2+ | 2.5 mM, 66% inhibition | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at a level twice the EDTA concentration completely restores enzyme activity | Streptococcus pneumoniae | |
Mg2+ | at a level twice the EDTA concentration completely restores enzyme activity | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.5 | - |
- |
Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycopeptide + H2O | release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages | Streptococcus pneumoniae | Galbeta(1-3)GalNAc + ? | - |
? | |
glycoprotein + H2O | release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages | Streptococcus pneumoniae | Galbeta(1-3)GalNAc + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptococcus pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | about 50% of maximal activity at pH 5.5 and at pH 7.0 | Streptococcus pneumoniae |