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Literature summary for 3.2.1.96 extracted from
- Characterization of novel endo-?-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG (2018), Sci. Rep., 8, 246 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21-CodonPlus (DE3) | Sphingobacterium sp. |
| expression in Escherichia coli BL21-CodonPlus (DE3) | Beauveria bassiana |
| expression in Escherichia coli BL21-CodonPlus (DE3) | Cordyceps militaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beauveria bassiana | J4WAU5 | - |
- |
| Beauveria bassiana ARSEF 2860 | J4WAU5 | - |
- |
| Cordyceps militaris | G3JPF7 | - |
- |
| Cordyceps militaris CM01 | G3JPF7 | - |
- |
| Sphingobacterium sp. | A0A2Z5VGL1 | - |
- |
| Sphingobacterium sp. | A0A2Z5VGM9 | - |
- |
| Sphingobacterium sp. | A0A2Z5VGN0 | - |
- |
| Sphingobacterium sp. | A0A2Z5VGV8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sphingobacterium sp. |
- |
Beauveria bassiana |
- |
Cordyceps militaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specicically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted | Sphingobacterium sp. | ? | - |
? |  |
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specicically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted | Cordyceps militaris | ? | - |
? | |
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specicically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted as a substrate | Beauveria bassiana | ? | - |
? | |
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specifically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted | Sphingobacterium sp. | ? | - |
? | |
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specicically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted as a substrate | Beauveria bassiana ARSEF 2860 | ? | - |
? | |
| additional information | the enzyme can hydrolytically remove fucose-containing oligosaccharides from glycoproteins. The enzyme specicically hydrolyzes fucose-containing biantennary oligosaccharides. It exhibits higher activity for the substrate containing terminal sialic acid with the alpha2,3-linkage. Terminal fucosylation is not accepted | Cordyceps militaris CM01 | ? | - |
? | |
| rituximab + H2O | releases the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B | Sphingobacterium sp. | ? | - |
? | |
| rituximab + H2O | releases the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B | Beauveria bassiana | ? | - |
? | |
| rituximab + H2O | releases the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B | Cordyceps militaris | ? | - |
? | |
| rituximab + H2O | releases the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B | Beauveria bassiana ARSEF 2860 | ? | - |
? | |
| rituximab + H2O | releases the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B | Cordyceps militaris CM01 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-N-acetylglucosaminidase | - |
Sphingobacterium sp. |
| endo-beta-N-acetylglucosaminidase | - |
Beauveria bassiana |
| endo-beta-N-acetylglucosaminidase | - |
Cordyceps militaris |
| ENGase | - |
Sphingobacterium sp. |
| ENGase | - |
Beauveria bassiana |
| ENGase | - |
Cordyceps militaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Sphingobacterium sp. |
| 2.5 | - |
- |
Sphingobacterium sp. |
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Release 2023.1 (January 2023)
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