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Literature summary for 3.2.1.96 extracted from

  • Nakamura, K.; Inoue, M.; Maeda, M.; Nakano, R.; Hosoi, K.; Fujiyama, K.; Kimura, Y.
    Molecular cloning and gene expression analysis of tomato endo-beta-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening (2009), Biosci. Biotechnol. Biochem., 73, 461-464.
    View publication on PubMed
Search for more literature for 3.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expressed from vector pET41-b in Escherichia coli BL21 (DE3) Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit expression of Endo-LE mRNA does not vary significantly with the tomato ripening process Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
Manalpha1-3Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
additional information recombinant Endo-LE, as well as native Endo-LE, is highly active towards high mannose type N-glycans with the Manalpha1-2Manalpha1-3Manbeta1-4GlcNAcbeta1-4GlcNAc unit Solanum lycopersicum ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
 Solanum lycopersicum
endo-LE
-
 Solanum lycopersicum
ENGase
-
 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Solanum lycopersicum