Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Glutamicibacter protophormiae |
Protein Variants | Comment | Organism |
---|---|---|
E173A | mutation in catalytically essential residue. Partial rescue of activity by addition of sodium azide or sodium formate, the produced beta-glycosyl azide retaines the anomeric configuration | Glutamicibacter protophormiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.207 | - |
dansyl-Asn-(GlcNAc)2(Man)5 | wild-type, pH 6.0, 37°C | Glutamicibacter protophormiae | |
0.876 | - |
dansyl-Asn-(GlcNAc)2(Man)5 | mutant E174A, presence of 2 M sodium azide, pH 6.0, 37°C | Glutamicibacter protophormiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glutamicibacter protophormiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-Asn-(GlcNAc)2(Man)5 + H2O | - |
Glutamicibacter protophormiae | dansyl-Asn-GlcNAc + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0458 | - |
dansyl-Asn-(GlcNAc)2(Man)5 | mutant E174A, presence of 2 M sodium azide, pH 6.0, 37°C | Glutamicibacter protophormiae | |
365 | - |
dansyl-Asn-(GlcNAc)2(Man)5 | wild-type, pH 6.0, 37°C | Glutamicibacter protophormiae |