Application | Comment | Organism |
---|---|---|
analysis | the enzyme will be an excellent tool for clarification of the structures and functions of complex oligosaccharides in glycoproteins | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 1 mM, 84% inhibition | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE | Pseudomonas sp. |
106000 | - |
x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
endo-beta-N-acetylglucosaminidase PI and PII | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Man)3(GlcNAc)2-N-Asn-dansyl + H2O | endo-beta-N-acetylglucosaminidase PII, no activity with endo-beta-N-acetylglucosaminidase PII | Pseudomonas sp. | ? | - |
? | |
additional information | endo-beta-N-acetylglucosaminidase PI hydrolyzes high-mannose type oligosaccharides GP-IV and GP-V faster than the hybrid type FP-I, GP-II, and GP-III. Endo-beta-N-acetylglucosaminidase PII hydrolyzes high mannose and hybrid type oligosaccharides | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE | Pseudomonas sp. |
? | x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 5% loss of activity, endo-beta-N-acetylglucosaminidase PII | Pseudomonas sp. |
50 | - |
10 min, 60% loss of activity, endo-beta-N-acetylglucosaminidase PII | Pseudomonas sp. |
55 | - |
10 min, stable, endo-beta-N-acetylglucosaminidase I | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | ENGase PI | Pseudomonas sp. |
6 | 8 | ENGase PII | Pseudomonas sp. |