Literature summary for 3.2.1.96 extracted from

Rao, V.; Cui, T.; Guan, C.; van Roey, P.
Mutations of endo-beta-N-acetylglucosaminidase H active site residues Asp130 and Glu132: activities and conformations (1999), Protein Sci., 8, 2338-2346.

Search for more literature for 3.2.1.96

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type enzyme and mutant enzymes D130N, D130E, D130A, E132Q, E132A, and D130N/E132Q	Streptomyces plicatus

Protein Variants

Protein Variants	Comment	Organism
D130	1.25-3.14% of the activity of the wild-type enzyme, depending on assay method	Streptomyces plicatus
D130A	0.04-0.1% of the activity of the wild-type enzyme	Streptomyces plicatus
D130N	0.1-0.21% of the activity of the wild-type enzyme, depending on assay method	Streptomyces plicatus
D130N/E132Q	no activity	Streptomyces plicatus
E132A	no activity	Streptomyces plicatus
E132D	0.03-0.05% of the activity of the wild-type enzyme	Streptomyces plicatus
E132Q	less than 0.05% of the activity of the wild-type enzyme	Streptomyces plicatus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces plicatus	-	mutant enzymes D130N, D130E, D130A, E132Q, E132A, and D130N/E132Q	-

Synonyms

Synonyms	Comment	Organism
endo-beta-N-acetylglucosaminidase H	-	Streptomyces plicatus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)