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Literature summary for 3.2.1.96 extracted from
- Purification and characterization of endo-beta-N-acetylglucosaminidase from hen oviduct (1997), J. Biochem., 122, 1167-1173.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Gallus gallus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
(Man)6(GlcNAc)2-pyridylamidated | - |
Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Gallus gallus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, SDS-PAGE in presence of 2-mercaptoethanol | Gallus gallus |
| 54000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Gallus gallus | in cytosol the enzyme contributes to the production of free oligosaccharides with one reducing end N-acetylglucosamine residue in cooperation with neutral alpha-mannosidase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
hen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oviduct | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.24 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, loss of activity within two to three days | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Man)6(GlcNAc)2-pyridylamidated + H2O | - |
Gallus gallus | ? | - |
? | |
| additional information | specificity | Gallus gallus | ? | - |
? | |
| additional information | the enzyme hydrolyzes oligomannose-type sugar chains faster than complex-type chains and hybrid-type chains. Sugar chains containing the manalpha1-2Manalpha1-3Manbeta1-4GlcNAcbeta1-GlcNAc structure are good substrates | Gallus gallus | ? | - |
? | |
| additional information | in cytosol the enzyme contributes to the production of free oligosaccharides with one reducing end N-acetylglucosamine residue in cooperation with neutral alpha-mannosidase | Gallus gallus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE in presence of 2-mercaptoethanol | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Endo-HO | - |
Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | pH 4.5: about 25% of maximal activity, pH 8.5: about 30% of maximal activity | Gallus gallus |
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