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Literature summary for 3.2.1.96 extracted from
- Purification and characterization of a novel fungal endo-beta-N-acetylglucosaminidase acting on complex oligosaccharides of glycoproteins (1990), Agric. Biol. Chem., 54, 97-106.
General Stability
| General Stability | Organism |
|---|---|
| activity decreases on repeated freezing | Mucor hiemalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 1 mM, significant inactivation | Mucor hiemalis |  |
| Hg2+ | 1 mM, significant inactivation | Mucor hiemalis | |
| PCMB | strong | Mucor hiemalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
dansyl asialotransferrin glycopeptide | - |
Mucor hiemalis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Mucor hiemalis |
| 95000 | - |
gel filtration | Mucor hiemalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucor hiemalis | - |
f. hiemalis | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mucor hiemalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Mucor hiemalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mucor hiemalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.0, 3 months, stable | Mucor hiemalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dansyl asialotransferrin glycopeptide + H2O | - |
Mucor hiemalis | ? | - |
? | |
| glycopeptide + H2O | high activity on dansyl ovalbumin glycopeptide, can also act on dansyl asialotransferrin glycopeptide containing biantennary type sugar chains | Mucor hiemalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Mucor hiemalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-N-acetylglucosaminidase M | - |
Mucor hiemalis |
| endo-M | - |
Mucor hiemalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Mucor hiemalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, 10 mM phosphate buffer, pH 7.0, stable up to | Mucor hiemalis |
| 55 | - |
10 min, 10 mM phosphate buffer, pH 7.0, 70% loss of activity | Mucor hiemalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Mucor hiemalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | 4°C, 3 days, stable | Mucor hiemalis |
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Release 2023.1 (January 2023)
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