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Literature summary for 3.2.1.96 extracted from

  • Trimble, R.B.; Tarentino, A.L.; Evans, G.; Maley, F.
    Purification and properties of endo-beta-N-acetylglucosaminidase L from Streptomyces plicatus (1979), J. Biol. Chem., 254, 9708-9713.
    View publication on PubMed
Search for more literature for 3.2.1.96

General Stability

General Stability Organism
20-30% loss of initial activity is lost when the protein concentration is decreased to less than 0.02-0.03 mg/ml Streptomyces plicatus
stable to lyophilization Streptomyces plicatus
stable to repeated freezing and thawing Streptomyces plicatus

Inhibitors

Inhibitors Comment Organism Structure
SDS 0.2%, complete inactivation Streptomyces plicatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 Man(GlcNAc)2Asn-dansyl
-
 Streptomyces plicatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Streptomyces plicatus
49500
-
 equilibrium sedimentation Streptomyces plicatus

Organism

Organism UniProt Comment Textmining
Streptomyces plicatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces plicatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces plicatus

Storage Stability

Storage Stability Organism
4°C, stable for up to 6 months Streptomyces plicatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn
-
 Streptomyces plicatus beta-D-Man-(1->4)-beta-D-GlcNAc + beta-D-GlcNAc-Asn
-
 ?
Man(GlcNAc)2Asn-dansyl + H2O
-
 Streptomyces plicatus ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Streptomyces plicatus 2 N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Streptomyces plicatus N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose
-
 ?
N4-(beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-N2-dansyl-L-Asn + H2O
-
 Streptomyces plicatus beta-D-Man-(1->4)-beta-D-GlcNAc + N4-(beta-D-GlcNAc)-N2-dansyl-L-Asn
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000-54000, SDS-PAGE Streptomyces plicatus

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase L
-
 Streptomyces plicatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 hydrolysis of Man(GlcNAc)2Asn-dansyl Streptomyces plicatus