Literature summary for 3.2.1.94 extracted from

Tochihara, T.; Sasaki, K.; Araki, O.; Morimoto, N.; Watanabe, K.; Hatada, Y.; Ito, S.; Ito, H.; Matsui, H.
Site-directed mutagenesis establishes aspartic acids-227 and -342 as essential for enzyme activity in an isomalto-dextranase from Arthrobacter globiformis (2004), Biotechnol. Lett., 26, 659-664.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Arthrobacter globiformis

Protein Variants

Protein Variants	Comment	Organism
D107A	activity similar to wild-type enzyme	Arthrobacter globiformis
D163A	activity similar to wild-type enzyme	Arthrobacter globiformis
D227A	no activity of dextran hydrolysis	Arthrobacter globiformis
D227E	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D227N	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D227Q	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D295A	activity similar to wild-type enzyme	Arthrobacter globiformis
D340A	activity similar to wild-type enzyme	Arthrobacter globiformis
D342A	no activity of dextran hydrolysis	Arthrobacter globiformis
D342E	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D342N	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D342Q	decreased activity of dextran hydrolysis	Arthrobacter globiformis
D373A	activity similar to wild-type enzyme	Arthrobacter globiformis
D396A	activity similar to wild-type enzyme	Arthrobacter globiformis
E420A	activity similar to wild-type enzyme	Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	SDS-PAGE	Arthrobacter globiformis

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter globiformis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
90% purity	Arthrobacter globiformis

Reaction

Reaction	Comment	Organism	Reaction ID
isomaltotriose + H2O = alpha-isomaltose + D-glucose	mechanism	Arthrobacter globiformis	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.012	-	E420A mutant	Arthrobacter globiformis
0.17	-	D396A mutant	Arthrobacter globiformis
0.22	-	D340A mutant	Arthrobacter globiformis
0.24	-	D373A mutant	Arthrobacter globiformis
0.35	-	D295A mutant	Arthrobacter globiformis
0.45	-	D163A mutant	Arthrobacter globiformis
0.67	-	D107A mutant	Arthrobacter globiformis
0.78	-	wild-type enzyme	Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dextran + H2O	-	Arthrobacter globiformis	alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc	-	?

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Release 2023.1 (January 2023)