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Literature summary for 3.2.1.94 extracted from
- Hyper-production of an isomalto-dextranase of an Arthrobacter sp. by a proteases-deficient Bacillus subtilis: sequencing, properties, and crystallization of the recombinant enzyme (2004), Appl. Microbiol. Biotechnol., 65, 583-592.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Bacillus subtilis | Arthrobacter dextranolyticus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method | Arthrobacter dextranolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 53% inactivation at 1 mM, 93% inactivation at 5 mM | Arthrobacter dextranolyticus |  |
| Fe3+ | 48% inactivation at 25 mM | Arthrobacter dextranolyticus | |
| Hg2+ | complete inactivation at 25 mM | Arthrobacter dextranolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
Dextran | 60°C, pH 3.5 | Arthrobacter dextranolyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | moderate activation | Arthrobacter dextranolyticus | |
| Li+ | moderate activation | Arthrobacter dextranolyticus | |
| Mn2+ | moderate activation | Arthrobacter dextranolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65500 | - |
SDS-PAGE | Arthrobacter dextranolyticus |
| 65990 | - |
calculated from amino acid sequence | Arthrobacter dextranolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter dextranolyticus | Q6BE01 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 95% pure | Arthrobacter dextranolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Arthrobacter dextranolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Arthrobacter dextranolyticus | alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMD | - |
Arthrobacter dextranolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Arthrobacter dextranolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable at pH 5 | Arthrobacter dextranolyticus |
| 60 | - |
60% activity at pH 5 after 30 min | Arthrobacter dextranolyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 496 | - |
Dextran | 60°C, pH 3.5 | Arthrobacter dextranolyticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Arthrobacter dextranolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 6 | 50% activity between pH 2.5 and pH 6 | Arthrobacter dextranolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10 | enzyme is stable at pH 4-8 and stable at pH 3.5-10 with 50% of the original activity | Arthrobacter dextranolyticus |
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