Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis | Arthrobacter dextranolyticus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Arthrobacter dextranolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 53% inactivation at 1 mM, 93% inactivation at 5 mM | Arthrobacter dextranolyticus | |
Fe3+ | 48% inactivation at 25 mM | Arthrobacter dextranolyticus | |
Hg2+ | complete inactivation at 25 mM | Arthrobacter dextranolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
Dextran | 60°C, pH 3.5 | Arthrobacter dextranolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | moderate activation | Arthrobacter dextranolyticus | |
Li+ | moderate activation | Arthrobacter dextranolyticus | |
Mn2+ | moderate activation | Arthrobacter dextranolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65500 | - |
SDS-PAGE | Arthrobacter dextranolyticus |
65990 | - |
calculated from amino acid sequence | Arthrobacter dextranolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter dextranolyticus | Q6BE01 | - |
- |
Purification (Comment) | Organism |
---|---|
95% pure | Arthrobacter dextranolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Arthrobacter dextranolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Arthrobacter dextranolyticus | alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMD | - |
Arthrobacter dextranolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Arthrobacter dextranolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable at pH 5 | Arthrobacter dextranolyticus |
60 | - |
60% activity at pH 5 after 30 min | Arthrobacter dextranolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
496 | - |
Dextran | 60°C, pH 3.5 | Arthrobacter dextranolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Arthrobacter dextranolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | 50% activity between pH 2.5 and pH 6 | Arthrobacter dextranolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | enzyme is stable at pH 4-8 and stable at pH 3.5-10 with 50% of the original activity | Arthrobacter dextranolyticus |