Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 80 | 110.12% activity at 1% (v/v) | Trichoderma virens |
Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus niger strain PY11 | Trichoderma virens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | competitive inhibitor | Trichoderma virens | |
Co2+ | 75.74% residual activity at 5 mM | Trichoderma virens | |
Cu2+ | 67.43% residual activity at 5 mM | Trichoderma virens | |
D-glucose | non-competitive inhibitor | Trichoderma virens | |
EDTA | 98.9% residual activity at 5 mM | Trichoderma virens | |
K+ | 90.66% residual activity at 5 mM | Trichoderma virens | |
Mg2+ | 68.12% residual activity at 5 mM | Trichoderma virens | |
Na+ | 96.61% residual activity at 5 mM | Trichoderma virens | |
Ni2+ | 70.55% residual activity at 5 mM | Trichoderma virens | |
SDS | 25.31% residual activity at 1% (w/v) | Trichoderma virens | |
Zn2+ | 91.5% residual activity at 5 mM | Trichoderma virens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.88 | - |
4-nitrophenyl beta-D-cellobioside | at pH 4.0 and 60°C | Trichoderma virens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 107.14% activity at 5 mM | Trichoderma virens | |
Mn2+ | 113.88% activity at 5 mM | Trichoderma virens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma virens | - |
- |
- |
Trichoderma virens UKM1 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has two potential N-glycosylation sites at Asn-283 and Asn-397 | Trichoderma virens |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and anion exchange column chromatography | Trichoderma virens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.51 | - |
crude enzyme, at pH 4.0 and 60°C | Trichoderma virens |
4.195 | - |
after 8.22fold purification, at pH 4.0 and 60°C | Trichoderma virens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Trichoderma virens | 4-methylumbelliferone + cellobiose | - |
? | |
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Trichoderma virens UKM1 | 4-methylumbelliferone + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Trichoderma virens | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Trichoderma virens UKM1 | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-lactopyranoside + H2O | - |
Trichoderma virens | 4-nitrophenol + beta-D-lactopyranose | - |
? | |
4-nitrophenyl beta-D-lactopyranoside + H2O | - |
Trichoderma virens UKM1 | 4-nitrophenol + beta-D-lactopyranose | - |
? | |
avicel + H2O | - |
Trichoderma virens | ? | - |
? | |
avicel + H2O | - |
Trichoderma virens UKM1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, recombinant enzyme, SDS-PAGE | Trichoderma virens |
? | x * 53280, calculated from amino acid sequence | Trichoderma virens |
Synonyms | Comment | Organism |
---|---|---|
CBH1 | - |
Trichoderma virens |
cellobiohydrolase I | - |
Trichoderma virens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma virens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme is stable for 1 h up to 60°C | Trichoderma virens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Trichoderma virens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | the enzyme is stable for 1 h at pH 3.0 to 6.0 in acetate buffer | Trichoderma virens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8.65 | - |
cellobiose | at pH 4.0 and 60°C | Trichoderma virens | |
24.61 | - |
D-glucose | at pH 4.0 and 60°C | Trichoderma virens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56800 | - |
4-nitrophenyl beta-D-cellobioside | at pH 4.0 and 60°C | Trichoderma virens |