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Literature summary for 3.2.1.91 extracted from

  • Heinzelman, P.; Snow, C.D.; Wu, I.; Nguyen, C.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
    A family of thermostable fungal cellulases created by structure-guided recombination (2009), Proc. Natl. Acad. Sci. USA, 106, 5610-5615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.91

Application

Application Comment Organism
synthesis SCHEMA structure-guided recombination of fungal class II cellobiohydrolases (CBH II cellulases) from Humicola insolens, Hypocrea jecorina and Chaetomium thermophiulum and mathematical modeling yields a collection of highly thermostable CBH II chimeras with more activity than Humicola insolens CBH II after incubation at 63 °C. The total of 15 validated thermostable CBH II enzymes have high sequence diversity, differing from their closest natural homologs at up to 63 amino acid positions. Selected purified thermostable chimeras hydrolyze phosphoric acid swollen cellulose at temperatures 7 to 15°C higher than the parent enzymes. These chimeras also hydrolyze as much or more cellulose than the parent CBH II enzymes in long-time cellulose hydrolysis assays and have pH/activity profiles as broad, or broader than, the parent enzymes. The best chimera with buildung blocks from all three organisms exhibits both relatively high specific activity and high thermostability Humicola insolens
synthesis SCHEMA structure-guided recombination of fungal class II cellobiohydrolases (CBH II cellulases) from Humicola insolens, Hypocrea jecorina and Chaetomium thermophiulum and mathematical modeling yields a collection of highly thermostable CBH II chimeras. The total of 15 validated thermostable CBH II enzymes have high sequence diversity, differing from their closest natural homologs at up to 63 amino acid positions. Selected purified thermostable chimeras hydrolyze phosphoric acid swollen cellulose at temperatures 7 to 15°C higher than the parent enzymes. These chimeras also hydrolyze as much or more cellulose than the parent CBH II enzymes in long-time cellulose hydrolysis assays and have pH/activity profiles as broad, or broader than, the parent enzymes. The best chimera with buildung blocks from all three organisms exhibits both relatively high specific activity and high thermostability Trichoderma reesei
synthesis SCHEMA structure-guided recombination of fungal class II cellobiohydrolases (CBH II cellulases) from Humicola insolens, Hypocrea jecorina and Chaetomium thermophiulum and mathematical modeling yields a collection of highly thermostable CBH II chimeras. The total of 15 validated thermostable CBH II enzymes have high sequence diversity, differing from their closest natural homologs at up to 63 amino acid positions. Selected purified thermostable chimeras hydrolyze phosphoric acid swollen cellulose at temperatures 7 to 15°C higher than the parent enzymes. These chimeras also hydrolyze as much or more cellulose than the parent CBH II enzymes in long-time cellulose hydrolysis assays and have pH/activity profiles as broad, or broader than, the parent enzymes. The best chimera with buildung blocks from all three organisms exhibits both relatively high specific activity and high thermostability Thermochaetoides thermophila

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Trichoderma reesei
expression in Saccharomyces cerevisiae Humicola insolens
expression in Saccharomyces cerevisiae Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Humicola insolens Q9C1S9
-
-
Thermochaetoides thermophila Q4JQF8
-
-
Trichoderma reesei P07987
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild-type, 13.0 microg glucose reducing sugar equivalent per microg enzyme and min, chimera HJPlus, 10.0 microg glucose reducing sugar equivalent per microg enzyme and min, pH 4.8, 50°C Trichoderma reesei
additional information
-
 wild-type, 13.0 microg glucose reducing sugar equivalent per microg enzyme and min, chimera HJPlus, 5.0 microg glucose reducing sugar equivalent per microg enzyme and min, pH 4.8, 50°C Thermochaetoides thermophila
additional information
-
 wild-type, 4.0 microg glucose reducing sugar equivalent per microg enzyme and min, chimera HJPlus, 10.0 microg glucose reducing sugar equivalent per microg enzyme and min, pH 4.8, 50°C Humicola insolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoric acid swollen cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Humicola insolens cellobiose + ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?

Synonyms

Synonyms Comment Organism
CBH2
-
 Trichoderma reesei
CBH2
-
 Thermochaetoides thermophila
Cel6A
-
 Humicola insolens