Application | Comment | Organism |
---|---|---|
degradation | application of recombinant CBH II in hydrolysis of corn stover and rice straw pretreated with sodium hydroxide by improving the exo-exosynergism between CBH II and CBH I in Hypocrea jecorina. The yields 94.7% and 83.3% are achieved in the enzymatic hydrolysis of corn stover and rice straw pretreated by sodium hydroxide, respectively | Trichoderma reesei |
synthesis | expression in Pichia pastoris after codon optimization and using the strong methanol-inducible promoter AOX1. 5.84 U CBH II per ml can be obtained at 96 h | Trichoderma reesei |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Trichoderma reesei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE, recombinant protein | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | P07987 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
microcrystalline cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
pretreated corn-stover + H2O | - |
Trichoderma reesei | ? | - |
? | |
pretreated rice straw + H2O | - |
Trichoderma reesei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE, recombinant protein | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
CBH II | - |
Trichoderma reesei |
CBH2 | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant protein | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant protein | Trichoderma reesei |