Cloned (Comment) | Organism |
---|---|
- |
Trichoderma reesei |
Crystallization (Comment) | Organism |
---|---|
- |
Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
D175A | mutant retains some activity on longer oligosaccharides | Trichoderma reesei |
D221A | almost complete loss of activity | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
cellopentaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
1.4 | - |
cellopentaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
2.6 | - |
cellotetraose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
4 | - |
cellotetraose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
14 | - |
cellohexaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
17 | - |
cellotriose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | P07987 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellohexaose + H2O | - |
Trichoderma reesei | ? | - |
? | |
cellopentaose + H2O | - |
Trichoderma reesei | ? | - |
? | |
cellotetraose + H2O | - |
Trichoderma reesei | ? | - |
? | |
cellotriose + H2O | - |
Trichoderma reesei | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
cellotriose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
0.0013 | - |
cellotetraose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
0.02 | - |
cellohexaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
0.025 | - |
cellopentaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
0.06 | - |
cellotriose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
1.1 | - |
cellopentaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
4.1 | - |
cellotetraose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
14 | - |
cellohexaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei |