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Literature summary for 3.2.1.89 extracted from

  • de Lima, E.A.; Machado, C.B.; Zanphorlin, L.M.; Ward, R.J.; Sato, H.H.; Ruller, R.
    GH53 endo-beta-1,4-galactanase from a newly isolated Bacillus licheniformis CBMAI 1609 as an enzymatic cocktail supplement for biomass saccharification (2016), Appl. Biochem. Biotechnol., 179, 415-426 .
    View publication on PubMed
Search for more literature for 3.2.1.89

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ about 5% residual activity at 5 mM Bacillus licheniformis
EDTA about 60% residual activity at 5 mM Bacillus licheniformis
EGTA about 80% residual activity at 5 mM Bacillus licheniformis
Fe3+ about 2% residual activity at 5 mM Bacillus licheniformis
Zn2+ about 5% residual activity at 5 mM Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 110% activity at 5 mM Bacillus licheniformis
Co2+ about 130% activity at 5 mM Bacillus licheniformis
K+ about 128% activity at 5 mM Bacillus licheniformis
Li+ about 118% activity at 5 mM Bacillus licheniformis
Mg2+ about 103% activity at 5 mM Bacillus licheniformis
Mn2+ about 115% activity at 5 mM Bacillus licheniformis
Na+ about 138% activity at 5 mM Bacillus licheniformis
Ni2+ about 145% activity at 5 mM Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus licheniformis CBMAI 1609
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating affinity column chromatography and Superdex G200 gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus fruit pectin + H2O
-
 Bacillus licheniformis ?
-
 ?
citrus fruit pectin + H2O
-
 Bacillus licheniformis CBMAI 1609 ?
-
 ?
potato galactan + H2O
-
 Bacillus licheniformis galactotetraose
-
 ?
potato galactan + H2O
-
 Bacillus licheniformis CBMAI 1609 galactotetraose
-
 ?
sugarcane bagasse + H2O
-
 Bacillus licheniformis ?
-
 ?
sugarcane bagasse + H2O
-
 Bacillus licheniformis CBMAI 1609 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48300, calculated from amino acid sequence Bacillus licheniformis

Synonyms

Synonyms Comment Organism
GAL
-
 Bacillus licheniformis
GH53 endo-beta-1,4-galactanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 about 20% activity at 20°C, about 50% activity at 30°C, 100% activity at 40°C, about 70% activity at 45°C, no activity at 50°C Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 the enzyme retains nearly 100% activity after 1 h at pH 7.0 at 25-45°C, The melting temperature is 52.9°C Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
555.8
-
 potato galactan at pH 6.0 and 40°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 the enzyme shows maximum activity (more than 90 %) in the pH range over 6.5 to 8.0 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the enzyme retains 75% activity at pH values 6.0 and 9.0 Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the enzyme retains more than 75 % activity after 24 h at pH 4.0-10.0 Bacillus licheniformis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus licheniformis calculated from amino acid sequence
-
 5.9