Cloned (Comment) | Organism |
---|---|
gene Pcgal1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha | Penicillium chrysogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Penicillium chrysogenum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
78000 | - |
x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | B5MGR3 | gene Pcgal1 | - |
Penicillium chrysogenum 31B | B5MGR3 | gene Pcgal1 | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from the culture supernatant by ammonium sulfate fractionation, ultrafiltration, anion exchange and hydrophobic interaction chromatography, and gel filtration | Penicillium chrysogenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
purified recombinant enzyme, substrate lupin galactan, pH 5.0, 37°C | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
debranched sugar beet arabinan + H2O | low activity | Penicillium chrysogenum | ? | - |
? | |
debranched sugar beet arabinan + H2O | low activity | Penicillium chrysogenum 31B | ? | - |
? | |
lupin beta-1,4-galactan + H2O | best substrate | Penicillium chrysogenum | ? | - |
? | |
lupin beta-1,4-galactan + H2O | best substrate | Penicillium chrysogenum 31B | ? | - |
? | |
additional information | substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside | Penicillium chrysogenum | ? | - |
? | |
additional information | substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside | Penicillium chrysogenum 31B | ? | - |
? | |
soybean arabinogalactan + H2O | high activity | Penicillium chrysogenum | ? | - |
? | |
soybean arabinogalactan + H2O | high activity | Penicillium chrysogenum 31B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-galactanase | - |
Penicillium chrysogenum |
PcGAL1 | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Penicillium chrysogenum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, pH 5.0, over 80% activity remaining after 1 h | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium chrysogenum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified recombinant enzyme, over 80% activity remaining after 16 h | Penicillium chrysogenum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 53, GH53 | Penicillium chrysogenum |
additional information | the enzyme acts synergistically with the exo-beta-1,4-galactanase of Penicillium chrysogenum | Penicillium chrysogenum |