Literature summary for 3.2.1.89 extracted from

Ryttersgaard, C.; Le Nours, J.; Lo Leggio, L.; Jorgensen, C.T.; Christensen, L.L.; Bjornvad, M.; Larsen, S.
The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products (2004), J. Mol. Biol., 341, 107-117.

Search for more literature for 3.2.1.89

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Bacillus subtilis	Bacillus licheniformis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme free or in complex with products galactobiose and galactotriose, hanging drop vapour diffusion method, 0.002-0.004 ml of protein solution, containing 20-40 mg/ml protein, are mixed with 0.002 ml reservoir solution containing 21-26% w/v PEG 1500, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement modelling	Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
beta-1,4-galactan + H2O	Bacillus licheniformis	plant-cell-wall-degrading enzyme involved in the hydrolysis of beta-1,4-galactan in the hairy regions of pectin	galactobiose + galactotriose	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	Q65CX5	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Bacillus subtilis by adsorption chromatography	Bacillus licheniformis

Reaction

Reaction	Comment	Organism	Reaction ID
Galbeta(1-6)Galbeta(1-4)(L-Arafalpha(1-3))Galbeta(1-4)Galbeta(1-4)(Galbeta(1-3)Galbeta(1-3)Galbeta + H2O = Galbeta(1-6)Galbeta + L-Arafalpha(1-3)Galbeta(1-4)Galbeta(1-4)(Galbeta(1-3)Galbeta(1-3)Galbeta	active site and substrate binding structures, overview	Bacillus licheniformis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-1,4-galactan + H2O	plant-cell-wall-degrading enzyme involved in the hydrolysis of beta-1,4-galactan in the hairy regions of pectin	Bacillus licheniformis	galactobiose + galactotriose	-	?
beta-1,4-galactan + H2O	1% w/v galactan from lupin treated with arabinofuranosidase	Bacillus licheniformis	galactobiose + galactotriose	product identification	?
additional information	no activity with galactobiose and galactotriose as substrates	Bacillus licheniformis	?	-	?

Subunits

Subunits	Comment	Organism
More	secondary and three-dimensional structure modelling and analysis, the enzyme structure shows a(betaalpha)8-barrel architecture and a calcium ion linking the long betaalpha-loops 7 and 8	Bacillus licheniformis

Synonyms

Synonyms	Comment	Organism
BLGAL	-	Bacillus licheniformis
endo-beta-1,4-galactanase	-	Bacillus licheniformis
More	the enzyme belongs to the glycoside hydrolase family 53, GH-53	Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Bacillus licheniformis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Bacillus licheniformis

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Release 2023.1 (January 2023)