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Literature summary for 3.2.1.89 extracted from

  • Le Nours, J.; Ryttersgaard, C.; Lo Leggio, L.; Ostergaard, P.R.; Borchert, T.V.; Christensen, L.L.H.; Larsen, S.
    Structure of two fungal beta-1,4-galactanases: Searching for the basis for temperature and pH optimum (2003), Protein Sci., 12, 1195-1204.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.89

Cloned(Commentary)

Cloned (Comment) Organism
-
 Humicola insolens
-
 Thermothelomyces heterothallicus
-
 Aspergillus aculeatus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Humicola insolens
hanging drop vapor diffusion method Thermothelomyces heterothallicus
hanging drop vapor diffusion method Aspergillus aculeatus

Protein Variants

Protein Variants Comment Organism
A90S/H91D decreased pH optimum Thermothelomyces heterothallicus
D128N increased pH optimum Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-
Humicola insolens
-
-
-
Thermothelomyces heterothallicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Humicola insolens
-
 Thermothelomyces heterothallicus
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azurine cross-linked galactan + H2O enzyme releases blue color Aspergillus aculeatus ?
-
 ?
azurine cross-linked lupin-galactan + H2O enzyme releases blue color Humicola insolens ?
-
 ?
azurine cross-linked lupin-galactan + H2O enzyme releases blue color Thermothelomyces heterothallicus ?
-
 ?
beta-1,4-galactan + H2O enzyme releases sugars from galactan Humicola insolens ?
-
 ?
beta-1,4-galactan + H2O enzyme releases sugars from galactan Thermothelomyces heterothallicus ?
-
 ?
beta-1,4-galactan + H2O enzyme releases sugars from lupin-galactan Aspergillus aculeatus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 at pH 6.5 Aspergillus aculeatus
50
-
 at pH 3.5 Aspergillus aculeatus
65
-
-
 Humicola insolens
65
-
-
 Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 wild-type enzyme Aspergillus aculeatus
4.5
-
 D128N mutant Aspergillus aculeatus
6.5
-
 A90S/H91D mutant Thermothelomyces heterothallicus
7.5
-
 wild-type enzyme Thermothelomyces heterothallicus
8.5
-
-
 Humicola insolens

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 at least 50% activity Aspergillus aculeatus
5.5 8.5 at least 50% activity Thermothelomyces heterothallicus
6.5 9.5 at least 50% activity Humicola insolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 5.5 at least 50% activity after 2 h at 37°C Aspergillus aculeatus
3.5 8.5 at least 50% activity after 2 h at 37°C Humicola insolens