Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Aspergillus aculeatus |
38000 | - |
SDS-PAGE, gel filtration | Aspergillus aculeatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus aculeatus | activity with galactans from different sources, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus aculeatus | - |
- |
- |
Aspergillus aculeatus | - |
from a commercial product Pectinex Ultra SP-L | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no glycoprotein | Aspergillus aculeatus |
Purification (Comment) | Organism |
---|---|
from a commercial product Pectinex Ultra SP-L | Aspergillus aculeatus |
native enzyme from commercial pectinase preparation by anion and cation exchange chromatography, ultrafiltration, and gel filtration | Aspergillus aculeatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | commercial pectinase preparation | Aspergillus aculeatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3059 | - |
purified enzyme | Aspergillus aculeatus |
3060 | - |
- |
Aspergillus aculeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-galactan + H2O | enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose | Aspergillus aculeatus | ? | - |
? | |
galactan I + H2O | isolated from black liquor, enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose | Aspergillus aculeatus | ? | - |
? | |
additional information | enzyme liberates galactose from a lignin-carbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-linked galactan | Aspergillus aculeatus | ? | - |
? | |
additional information | activity with galactans from different sources, overview | Aspergillus aculeatus | ? | - |
? | |
additional information | the enzyme is highly active and specific for beta-galactans hydrolyzing beta-1,4-galactosidic linkages, substrate specificity, the enzyme acts on beta-1,4-linked galactan, producing a range of galacto-oligosaccharides, and on beta-1,4- and beta-1,3/6-linked arabinogalactans, the enzyme liberates galactose from a lignincarbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-liked galactan, overview | Aspergillus aculeatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Aspergillus aculeatus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-galactanase | - |
Aspergillus aculeatus |
endo-beta-1,4-D-galactanase | - |
Aspergillus aculeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aspergillus aculeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
up to, purified enzyme, pH 4.5, 24 h, 80% remaining activity | Aspergillus aculeatus |
45 | - |
purified enzyme, pH 4.5, 24 h, 47% remaining activity | Aspergillus aculeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus aculeatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.4 | 5 | enzyme acts efficiently between pH 3.4 and pH 5 | Aspergillus aculeatus |
3.4 | 5 | high activity within this range | Aspergillus aculeatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.2 | 7.5 | enzyme retains at least 80% of its activity between pH 4.4 and pH 7 at 40°C after 24 h, but loses 50% of its activity below pH 3.2 and above pH 7.5 | Aspergillus aculeatus |
3.2 | 7.5 | purified enzyme, 24 h, 40°C, 80% remaining activity at pH 4.4 and pH 7.0, less than 50% remaining activity below pH 3.2 and above pH 7.5 | Aspergillus aculeatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus aculeatus | below, isoelectric focusing | - |
4 |
Aspergillus aculeatus | pI below 4, chromatofocusing | - |
4 |