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Literature summary for 3.2.1.89 extracted from

  • Luonteri, E.; Laine, C.; Uusitalo, S.; Teleman, A.; Siika-aho, M.; Tenkanen, M.
    Purification and characterization of Aspergillus beta-D-galactanases acting on beta-1,4- and beta-1,3/6-linked arabinogalactans (2003), Carbohydr. Polym., 53, 155-168.
No PubMed abstract available
Search for more literature for 3.2.1.89

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Aspergillus aculeatus
38000
-
 SDS-PAGE, gel filtration Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus aculeatus activity with galactans from different sources, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-
Aspergillus aculeatus
-
 from a commercial product Pectinex Ultra SP-L
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Aspergillus aculeatus

Purification (Commentary)

Purification (Comment) Organism
from a commercial product Pectinex Ultra SP-L Aspergillus aculeatus
native enzyme from commercial pectinase preparation by anion and cation exchange chromatography, ultrafiltration, and gel filtration Aspergillus aculeatus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation commercial pectinase preparation Aspergillus aculeatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3059
-
 purified enzyme Aspergillus aculeatus
3060
-
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-galactan + H2O enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose Aspergillus aculeatus ?
-
 ?
galactan I + H2O isolated from black liquor, enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose Aspergillus aculeatus ?
-
 ?
additional information enzyme liberates galactose from a lignin-carbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-linked galactan Aspergillus aculeatus ?
-
 ?
additional information activity with galactans from different sources, overview Aspergillus aculeatus ?
-
 ?
additional information the enzyme is highly active and specific for beta-galactans hydrolyzing beta-1,4-galactosidic linkages, substrate specificity, the enzyme acts on beta-1,4-linked galactan, producing a range of galacto-oligosaccharides, and on beta-1,4- and beta-1,3/6-linked arabinogalactans, the enzyme liberates galactose from a lignin–carbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-liked galactan, overview Aspergillus aculeatus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Aspergillus aculeatus

Synonyms

Synonyms Comment Organism
beta-D-galactanase
-
 Aspergillus aculeatus
endo-beta-1,4-D-galactanase
-
 Aspergillus aculeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 up to, purified enzyme, pH 4.5, 24 h, 80% remaining activity Aspergillus aculeatus
45
-
 purified enzyme, pH 4.5, 24 h, 47% remaining activity Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
3.4 5 enzyme acts efficiently between pH 3.4 and pH 5 Aspergillus aculeatus
3.4 5 high activity within this range Aspergillus aculeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2 7.5 enzyme retains at least 80% of its activity between pH 4.4 and pH 7 at 40°C after 24 h, but loses 50% of its activity below pH 3.2 and above pH 7.5 Aspergillus aculeatus
3.2 7.5 purified enzyme, 24 h, 40°C, 80% remaining activity at pH 4.4 and pH 7.0, less than 50% remaining activity below pH 3.2 and above pH 7.5 Aspergillus aculeatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus aculeatus below, isoelectric focusing
-
 4
Aspergillus aculeatus pI below 4, chromatofocusing
-
 4