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Literature summary for 3.2.1.88 extracted from

  • Wefers, D.; Dong, J.; Abdel-Hamid, A.M.; Paul, H.M.; Pereira, G.V.; Han, Y.; Dodd, D.; Baskaran, R.; Mayer, B.; Mackie, R.I.; Cann, I.
    Enzymatic mechanism for arabinan degradation and transport in the thermophilic bacterium Caldanaerobius polysaccharolyticus (2017), Appl. Environ. Microbiol., 83, e00794-17 .
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 4-nitrophenyl beta-L-arabinopyranoside at pH 5.5 and 60°C Caldanaerobius polysaccharolyticus

Organism

Organism UniProt Comment Textmining
Caldanaerobius polysaccharolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-L-arabinopyranoside + H2O highest activity Caldanaerobius polysaccharolyticus 4-nitrophenol + beta-L-arabinopyranose
-
 ?
arabinan + H2O very low activity Caldanaerobius polysaccharolyticus ?
-
 ?
debranched arabinan + H2O low activity Caldanaerobius polysaccharolyticus ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside and arabino-oligosaccharide A-5b (composed of an alpha-1,5-linked trisaccharide replaced with a beta-1,3-linked arabinobiose side chain at position O-3) Caldanaerobius polysaccharolyticus ?
-
 ?

Synonyms

Synonyms Comment Organism
Abp27A
-
 Caldanaerobius polysaccharolyticus
beta-arabinopyranosidase
-
 Caldanaerobius polysaccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Caldanaerobius polysaccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 70 the enzyme retains most of its activity at 65°C and 70°C for 1 h, but longer incubation times at all temperatures lead to drastic decreases of activity. After 10 min at 80°C, the enzyme shows at least 75% activity Caldanaerobius polysaccharolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.8
-
 4-nitrophenyl beta-L-arabinopyranoside at pH 5.5 and 60°C Caldanaerobius polysaccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Caldanaerobius polysaccharolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6
-
 4-nitrophenyl beta-L-arabinopyranoside at pH 5.5 and 60°C Caldanaerobius polysaccharolyticus