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Literature summary for 3.2.1.86 extracted from

  • Wilson, G.; Fox, C.F.
    The beta-glucoside system of Escherichia coli. IV. Purification and properties of phospho-beta-glucosidases A and B (1974), J. Biol. Chem., 249, 5586-5598.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP
-
Escherichia coli
3',5'-AMP
-
Escherichia coli
3'-AMP
-
Escherichia coli
AMP
-
Escherichia coli
ATP
-
Escherichia coli
CMP
-
Escherichia coli
DL-alpha-glycerol-phosphate
-
Escherichia coli
fructose-1,6-diphosphate
-
Escherichia coli
NADH
-
Escherichia coli
phosphoenolpyruvate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
4-nitrothiophenyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
0.18
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate isoenzyme B Escherichia coli
0.19
-
methyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
0.25
-
2-nitrophenyl beta-D-galactoside 6-phosphate isoenzyme B Escherichia coli
0.28
-
methyl beta-D-glucoside 6-phosphate isoenzyme A Escherichia coli
0.33
-
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate isoenzyme A Escherichia coli
0.46
-
phenyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
0.51
-
Salicin 6-phosphate isoenzyme B Escherichia coli
0.6
-
phenyl beta-D-glucoside 6-phosphate isoenzyme A Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
isoenzyme B, SDS-PAGE Escherichia coli
68000
-
isoenzyme A, SDS-PAGE Escherichia coli
132000
-
isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement Escherichia coli
142000
-
isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-

Purification (Commentary)

Purification (Comment) Organism
63 fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
isoenzyme A, 4°C, 1 year, sterile starting buffer, 0% loss in activity Escherichia coli
isoenzyme B, -20°C, 2 months, sterile starting buffer, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactoside 6-phosphate + H2O
-
Escherichia coli 2-nitrophenol + beta-D-galactose 6-phosphate
-
?
4-nitrothiophenyl beta-D-glucoside 6-phosphate + H2O
-
Escherichia coli 4-nitrothiophenol + beta-D-glucose 6-phosphate
-
?
methyl beta-glucoside 6-phosphate + H2O
-
Escherichia coli ?
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Escherichia coli o-nitrophenol + D-galactose 6-phosphate
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O isoenzyme B Escherichia coli p-nitrophenol + D-glucopyranose 6-phosphate
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
-
Escherichia coli ?
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
-
Escherichia coli phenol + glucose 6-phosphate
-
?
salicin 6-phosphate + H2O isoenzyme B Escherichia coli 2-hydroxymethylphenol + D-glucose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 28 isoenzyme B Escherichia coli
25 45 isoenzyme A Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
isoenzyme A, p-nitrophenyl beta-D-glucospyranoside Escherichia coli
7 9 isoenzyme B, p-nitrophenyl beta-D-glucospyranoside Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 for more than 1 h Escherichia coli