Crystallization (Comment) | Organism |
---|---|
in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E170Q | catalytic mutant | Geobacillus stearothermophilus |
W433A | the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity | Geobacillus stearothermophilus |
W433M | the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | W8QF82 | - |
- |
Geobacillus stearothermophilus T-1 | W8QF82 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose-6-phosphate + H2O | - |
Geobacillus stearothermophilus | D-glucose-6-phosphate + D-glucose | - |
? | |
cellobiose-6-phosphate + H2O | - |
Geobacillus stearothermophilus T-1 | D-glucose-6-phosphate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55204, calculated from amino acid sequence | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
Gan1D | the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities | Geobacillus stearothermophilus |
Organism | Comment | Expression |
---|---|---|
Geobacillus stearothermophilus | the enzyme expression is upregulated by D-galactose | up |