Cloned (Comment) | Organism |
---|---|
LacG1, phylogenetic analysis | Lactobacillus gasseri |
LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363 | Lactobacillus gasseri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 6.0, 40°C | Lactobacillus gasseri | |
5.5 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 5.5, 50°C | Lactobacillus gasseri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE | Lactobacillus gasseri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | O06532 | - |
- |
Lactobacillus gasseri ATCC 33323 | O06532 | - |
- |
Purification (Comment) | Organism |
---|---|
native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration | Lactobacillus gasseri |
recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration | Lactobacillus gasseri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri | 2-nitrophenol + beta-D-galactose 6-phosphate | - |
? | |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri ATCC 33323 | 2-nitrophenol + beta-D-galactose 6-phosphate | - |
? | |
additional information | the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86 | Lactobacillus gasseri | ? | - |
? | |
additional information | the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86 | Lactobacillus gasseri ATCC 33323 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant enzyme, SDS-PAGE | Lactobacillus gasseri |
Synonyms | Comment | Organism |
---|---|---|
LacG1 | - |
Lactobacillus gasseri |
LacG2 | - |
Lactobacillus gasseri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactobacillus gasseri |
50 | - |
- |
Lactobacillus gasseri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
206 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 6.0, 40°C | Lactobacillus gasseri | |
361 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 5.5, 50°C | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Lactobacillus gasseri |
6 | - |
- |
Lactobacillus gasseri |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyms is involved in the metabolic pathway of lactose in Lactbacillus gasseri, overview | Lactobacillus gasseri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 6.0, 40°C | Lactobacillus gasseri | |
93 | - |
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 6.0, 40°C | Lactobacillus gasseri |