Literature summary for 3.2.1.85 extracted from

Lansky, S.; Zehavi, A.; Dann, R.; Dvir, H.; Belrhali, H.; Shoham, Y.; Shoham, G.
Purification, crystallization and preliminary crystallographic analysis of Gan1D, a GH1 6-phospho-beta-galactosidase from Geobacillus stearothermophilus T1 (2014), Acta Crystallogr. Sect. F, 70, 225-231.

Cloned(Commentary)

Cloned (Comment)	Organism
gene gan1D, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged wild-type enzyme, hanging drop vapour diffusion method, two conditions and two crystal forms: 1. mixing of 3.9-4.3 mg/ml protein with reservoir solution containing 16-18% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.5 in a 1:1 ratio, or 2. mixing of 4.3-4.5 mg/ml protein with reservoir solution containing 19-22% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.48, in a 1:1 ratio, X-ray diffraction structure determination and analysis at 1.33-1.92 A resolution, detailed three-dimensional crystal structure analysis, overview	Geobacillus stearothermophilus

Crystallization (Comment)

Organism

purified recombinant His-tagged wild-type enzyme, hanging drop vapour diffusion method, two conditions and two crystal forms: 1. mixing of 3.9-4.3 mg/ml protein with reservoir solution containing 16-18% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.5 in a 1:1 ratio, or 2. mixing of 4.3-4.5 mg/ml protein with reservoir solution containing 19-22% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.48, in a 1:1 ratio, X-ray diffraction structure determination and analysis at 1.33-1.92 A resolution, detailed three-dimensional crystal structure analysis, overview

Geobacillus stearothermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Geobacillus stearothermophilus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	recombinant His-tagged enzyme, gel filtration	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	W8QF82	gene gan1D	-
Geobacillus stearothermophilus T1	W8QF82	gene gan1D	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
Gan1D	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	-	Geobacillus stearothermophilus

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 1, GH1	Geobacillus stearothermophilus