Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.85 extracted from

  • Schulte, D.; Hengstenberg, W.
    Engineering the active center of the 6-phospho-beta-galactosidase from Lactococcus lactis (2000), Protein Eng., 13, 515-518.
    View publication on PubMed
Search for more literature for 3.2.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K12 TG1 Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
K435V/Y437F significantly reduced substrate binding and turnover Lactococcus lactis
S428D/K435V/Y437F no activity Lactococcus lactis
W429A improved specificity for glucosides Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 o-nitrophenyl-beta-D-galactoside wild type enzyme, pH 7.5, room temperature Lactococcus lactis
0.39
-
 p-nitrophenyl-beta-D-galactopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
0.54
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
0.57
-
 o-nitrophenyl-beta-D-galactoside K435V/Y437F mutant enzyme, pH 7.5, room temperature Lactococcus lactis
1.56
-
 o-nitrophenyl-beta-D-glucopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
2.86
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
2.99
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.1
-
 p-nitrophenyl-beta-D-galactopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
4.89
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate K435V/Y437F mutant enzyme, pH 7.5, room temperature Lactococcus lactis
9.83
-
 o-nitrophenyl-beta-D-glucopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
21.53
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose-6-phosphate + H2O Lactococcus lactis involved in lactose assimilation D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P11546
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose-6-phosphate + H2O
-
 Lactococcus lactis D-glucose + beta-D-galactose-6-phosphate
-
 ?
lactose-6-phosphate + H2O involved in lactose assimilation Lactococcus lactis D-glucose + beta-D-galactose-6-phosphate
-
 ?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-glucose-6-phosphate
-
 ?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O less effective than ortho-form Lactococcus lactis p-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O less effective than ortho-form Lactococcus lactis p-nitrophenol + beta-D-glucose-6-phosphate
-
 ?