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Literature summary for 3.2.1.85 extracted from

  • Suzuki, M.; Saito, T.; Itoh, T.
    Coexistence of two kinds of 6-phospho-beta galactosidase in the cytosol of Lactobacillus gasseri JCM 1031 - purification and characterization of 6-phospho-beta-galactosidase II (1996), Biosci. Biotechnol. Biochem., 60, 708-710.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol lower effect on P-beta-gal II than P-beta-gal I Lactobacillus gasseri
Fe2+ 60% increase of activity Lactobacillus gasseri

Inhibitors

Inhibitors Comment Organism Structure
D-Glucose-6-phosphate 63% of activity Lactobacillus gasseri
iodoacetamide
-
Lactobacillus gasseri
N-ethylmaleimide
-
Lactobacillus gasseri
p-chloromercuribenzoic acid
-
Lactobacillus gasseri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76 0.8 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactobacillus gasseri

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 78% of activity at 1 mM CaCl2 Lactobacillus gasseri
Hg2+ complete inhibition at 1 mM HgCl2 Lactobacillus gasseri
Mg2+ slight inhibition at 1 mM MgCl2 Lactobacillus gasseri
Mn2+ slight inhibition at 1 mM MnCl2 Lactobacillus gasseri
Zn2+ 98.4% inhibition at 1 mM ZnSO4 Lactobacillus gasseri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose-6-phosphate + H2O Lactobacillus gasseri
-
D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O Lactobacillus gasseri JCM 1031
-
D-glucose + beta-D-galactose-6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri
-
JCM 1031
-
Lactobacillus gasseri JCM 1031
-
JCM 1031
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose-6-phosphate + H2O
-
Lactobacillus gasseri D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O
-
Lactobacillus gasseri JCM 1031 D-glucose + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactobacillus gasseri o-nitrophenol + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactobacillus gasseri JCM 1031 o-nitrophenol + beta-D-galactose-6-phosphate
-
?

Synonyms

Synonyms Comment Organism
P-beta-gal I and II Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
P-beta-gal II Lactobacillus gasseri
50
-
P-beta-gal I Lactobacillus gasseri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 P-beta-gal II, stable below 40°C, inactivated at 60°C Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Lactobacillus gasseri

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9
-
Lactobacillus gasseri