Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | enhanced activiy after dialysis by addition up to 0.75 mM | Lactococcus cremoris | |
EDTA | slight stimulation to 118.2% by 2 mM | Lactococcus cremoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose-6-phosphate | competitive inhibition | Lactococcus cremoris | |
p-hydroxymercuribenzoate | 83.4% of control activity at 1 mM, 12.2% at 2 mM | Lactococcus cremoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactococcus cremoris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | 82% of control activity at 2 mM | Lactococcus cremoris | |
Mg2+ | 74.5% of control activity at 2 mM | Lactococcus cremoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67600 | - |
gel filtration | Lactococcus cremoris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | Lactococcus cremoris | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | Lactococcus cremoris HP | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus cremoris | - |
HP | - |
Lactococcus cremoris HP | - |
HP | - |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus cremoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.8 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate | Lactococcus cremoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | - |
Lactococcus cremoris | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | - |
Lactococcus cremoris HP | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus cremoris | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactococcus cremoris HP | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-phosphogalactoside galactohydrolase | - |
Lactococcus cremoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 52 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate | Lactococcus cremoris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable up to for 5 min | Lactococcus cremoris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | nearly constant activity | Lactococcus cremoris |