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Literature summary for 3.2.1.85 extracted from

  • Johnson, K.G.; McDonald, I.J.
    beta-D-Phosphogalactoside galactohydrolase from Streptococcus cremoris HP: purification and enzyme properties (1974), J. Bacteriol., 117, 667-674.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol enhanced activiy after dialysis by addition up to 0.75 mM Lactococcus cremoris
EDTA slight stimulation to 118.2% by 2 mM Lactococcus cremoris

Inhibitors

Inhibitors Comment Organism Structure
D-galactose-6-phosphate competitive inhibition Lactococcus cremoris
p-hydroxymercuribenzoate 83.4% of control activity at 1 mM, 12.2% at 2 mM Lactococcus cremoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactococcus cremoris

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 82% of control activity at 2 mM Lactococcus cremoris
Mg2+ 74.5% of control activity at 2 mM Lactococcus cremoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67600
-
gel filtration Lactococcus cremoris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose-6-phosphate + H2O Lactococcus cremoris
-
D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O Lactococcus cremoris HP
-
D-glucose + beta-D-galactose-6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus cremoris
-
HP
-
Lactococcus cremoris HP
-
HP
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus cremoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.8
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate Lactococcus cremoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose-6-phosphate + H2O
-
Lactococcus cremoris D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O
-
Lactococcus cremoris HP D-glucose + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactococcus cremoris o-nitrophenol + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactococcus cremoris HP o-nitrophenol + beta-D-galactose-6-phosphate
-
?

Synonyms

Synonyms Comment Organism
beta-D-phosphogalactoside galactohydrolase
-
Lactococcus cremoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 52 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate Lactococcus cremoris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable up to for 5 min Lactococcus cremoris

pH Range

pH Minimum pH Maximum Comment Organism
5 8 nearly constant activity Lactococcus cremoris