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Literature summary for 3.2.1.83 extracted from

  • Zhao, Y.; Chi, Z.; Xu, Y.; Shi, N.; Chi, Z.; Liu, G.
    High-level extracellular expression of kappa-carrageenase in Brevibacillus choshinensis for the production of a series of kappa-carrageenan oligosaccharides (2018), Process Biochem., 64, 83-92 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 102.1% activity at 10 mM Pseudoalteromonas porphyrae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Brevibacillus choshinensis Pseudoalteromonas porphyrae

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2.1% residual activity at 50 mM Pseudoalteromonas porphyrae
EDTA 97.4% residual activity at 10 mM Pseudoalteromonas porphyrae
EGTA 87.8% residual activity at 10 mM Pseudoalteromonas porphyrae
Fe2+ 8.1% residual activity at 50 mM Pseudoalteromonas porphyrae
Fe3+ 9.2% residual activity at 50 mM Pseudoalteromonas porphyrae
Hg2+ 0.3% residual activity at 50 mM Pseudoalteromonas porphyrae
Mn2+ 53.5% residual activity at 50 mM Pseudoalteromonas porphyrae
phenylmethylsulfonyl fluoride 94.3% residual activity at 10 mM Pseudoalteromonas porphyrae
SDS 1.8% residual activity at 10 mM Pseudoalteromonas porphyrae
Zn2+ 8.3% residual activity at 50 mM Pseudoalteromonas porphyrae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 100 mM Ca2+ enhances the activity of the enzyme by 38% Pseudoalteromonas porphyrae
K+ 100 mM K+ enhances the activity of the enzyme by 3% Pseudoalteromonas porphyrae
Mg2+ 100 mM Mg2+ enhances the activity of the enzyme by 97.5% Pseudoalteromonas porphyrae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Pseudoalteromonas porphyrae
-
kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose
-
?
kappa-carrageen + H2O Pseudoalteromonas porphyrae LL1
-
kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose
-
?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas porphyrae
-
-
-
Pseudoalteromonas porphyrae LL1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography and Sephacryl S-100 gel filtration Pseudoalteromonas porphyrae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
Pseudoalteromonas porphyrae kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose
-
?
kappa-carrageen + H2O
-
Pseudoalteromonas porphyrae LL1 kappa-neocarraoctaose + kappa-neocarrahexaose + kappa-neocarratetraose + kappa-neocarrabiose
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Pseudoalteromonas porphyrae
? x * 31500, calculated from amino acid sequence Pseudoalteromonas porphyrae

Synonyms

Synonyms Comment Organism
CGK
-
Pseudoalteromonas porphyrae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudoalteromonas porphyrae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 the enzyme shows more than 70% from 30 to 45°C Pseudoalteromonas porphyrae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 over 80% of the total activity is retained after 60 min at 40°C. When the incubation temperature rises above 45°C, the activity of the enzyme decreases rapidly. Furthermore, the enzyme is almost inactivated at 50°C within 60 min Pseudoalteromonas porphyrae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudoalteromonas porphyrae

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 50% activity between pH 6.0 and 9.0 Pseudoalteromonas porphyrae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 the enzyme is considerably stable from pH 7.0 to 9.0, where its activity declines by less than 10% Pseudoalteromonas porphyrae