Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.83 extracted from

  • Li, J.; Pan, A.; Xie, M.; Zhang, P.; Gu, X.
    Characterization of a thermostable kappa-carrageenase from a hot spring bacterium and plant protection activity of the oligosaccharide enzymolysis product (2019), J. Sci. Food Agric., 99, 1812-1819 .
    View publication on PubMed
Search for more literature for 3.2.1.83

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ about 180% activity at 2 mM Bacillus sp. HT19
Cu2+ about 110% activity at 2 mM Bacillus sp. HT19

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ about 80% residual activity at 2 mM Bacillus sp. HT19
EDTA about 90% residual activity at 2 mM Bacillus sp. HT19
Fe2+ about 70% residual activity at 2 mM Bacillus sp. HT19
Sr2+ about 50% residual activity at 2 mM Bacillus sp. HT19

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ni2+, Mg2+ and Mn2+ have almost no effect on the enzyme activity Bacillus sp. HT19

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Bacillus sp. HT19
-
 kappa-neocarrabiose
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. HT19
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Sephadex G200 gel filtration Bacillus sp. HT19

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
 Bacillus sp. HT19 kappa-neocarrabiose
-
 ?
additional information no activity with iota-carrageenan, lambda-carrageenan, agar, and sodium alginate Bacillus sp. HT19 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Bacillus sp. HT19

Synonyms

Synonyms Comment Organism
Car19
-
 Bacillus sp. HT19

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. HT19

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 about 40% activity at 40°C, about 90% activity at 50°C, 100% activity at 60°C, about 55% activity at 70°C Bacillus sp. HT19

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme retains more than 90% of its initial activity after incubation at 50-60°C for 24 h. The activity declines sharply after 60 min of incubation at 70°C Bacillus sp. HT19

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus sp. HT19

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 65% activity between pH 6.0 and 9.0 Bacillus sp. HT19