Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 98% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Cu2+ | 32% residual activity at 1 mM | Vibrio sp. NJ-2 | |
EDTA | 71% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Fe2+ | 51% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Fe3+ | 38% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Mn2+ | 39% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Ni2+ | 49% residual activity at 1 mM | Vibrio sp. NJ-2 | |
SDS | 56% residual activity at 1 mM | Vibrio sp. NJ-2 | |
Zn2+ | 21% residual activity at 1 mM | Vibrio sp. NJ-2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 108% activity at 1 mM | Vibrio sp. NJ-2 | |
K+ | 153% activity at 1 mM | Vibrio sp. NJ-2 | |
Mg2+ | 112% activity at 1 mM | Vibrio sp. NJ-2 | |
Na+ | 369% activity at 300 mM | Vibrio sp. NJ-2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | Vibrio sp. NJ-2 | - |
kappa-neocarraoctaose-sulfate + kappa-neocarrahexaose-sulfate + kappa-neocarratetraose-sulfate + kappa-neocarrabiose-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. NJ-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex 200 gel filtration, and Sephadex G-75 gel filtration | Vibrio sp. NJ-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Vibrio sp. NJ-2 | kappa-neocarraoctaose-sulfate + kappa-neocarrahexaose-sulfate + kappa-neocarratetraose-sulfate + kappa-neocarrabiose-sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Vibrio sp. NJ-2 |
Synonyms | Comment | Organism |
---|---|---|
CgkA | - |
Vibrio sp. NJ-2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vibrio sp. NJ-2 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme maintains more than 60% of total activity at 30-50°C | Vibrio sp. NJ-2 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | the enzyme maintains more than 90% of total activity after 30 min at 10-40°C. At 50°C, the activity drops to about 20% | Vibrio sp. NJ-2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Vibrio sp. NJ-2 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme maintains more than 70% of total activity between pH 6.0 and 9.0 | Vibrio sp. NJ-2 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme maintains 80% of total activity after 24 h between pH 6.0 and 10.0. At pH 5.0 and 11.0, the activity drops to about 55% and 2%, respectively | Vibrio sp. NJ-2 |