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Literature summary for 3.2.1.83 extracted from
- Cloning and biochemical characterization of a novel kappa-carrageenase from newly isolated marine bacterium Pedobacter hainanensis NJ-02 (2018), Int. J. Biol. Macromol., 108, 1331-1338 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pedobacter hainanensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 80% residual activity at 1 mM | Pedobacter hainanensis |  |
| EDTA | 86% residual activity at 1 mM | Pedobacter hainanensis | |
| Fe2+ | 72% residual activity at 1 mM | Pedobacter hainanensis | |
| K+ | 70% residual activity at 100 mM | Pedobacter hainanensis | |
| Mn2+ | 67% residual activity at 1 mM | Pedobacter hainanensis | |
| NH4+ | 82% residual activity at 1 mM | Pedobacter hainanensis | |
| SDS | 61% residual activity at 1 mM | Pedobacter hainanensis | |
| Zn2+ | 65% residual activity at 1 mM | Pedobacter hainanensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by Mg2+ | Pedobacter hainanensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pedobacter hainanensis | - |
- |
- |
| Pedobacter hainanensis NJ-02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA Sepharose column chromatography | Pedobacter hainanensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | - |
Pedobacter hainanensis | kappa-neocarrahexaose + kappa-neocarratetraose + D-galactose | - |
? | |
| kappa-carrageen + H2O | - |
Pedobacter hainanensis NJ-02 | kappa-neocarrahexaose + kappa-neocarratetraose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-PAGE | Pedobacter hainanensis |
| ? | x * 57120, calculated from amino acid sequence | Pedobacter hainanensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CgkA | - |
Pedobacter hainanensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pedobacter hainanensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | about 45% activity at 20°C, about 80% activity at 30°C, 100% activity at 40°C, about 65% activity at 50°C | Pedobacter hainanensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | the enzyme retains more than 90% activity after 3 h at 10-40°C | Pedobacter hainanensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pedobacter hainanensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | more than 70% activity at pH 8.0, more than 60% activity at pH 9.0 in Tris-HCl buffer | Pedobacter hainanensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | the enzyme retains more than 80% of its maximal activity after being incubated for 30 min at pH of 5.0-9.0 below 40°C | Pedobacter hainanensis |
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