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Literature summary for 3.2.1.83 extracted from
- Cloning, identification and characterization of a novel kappa-carrageenase from marine bacterium Cellulophaga lytica strain N5-2 (2017), Int. J. Biol. Macromol., 105, 509-515 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximum activity in the presence of 0.6% NaCl | Cellulophaga lytica |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Cellulophaga lytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 30.65% residual activity at 1 mM | Cellulophaga lytica | |
| K+ | 23.6% residual activity at 100 mM | Cellulophaga lytica | |
| SDS | 22.71% residual activity at 1 mM | Cellulophaga lytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by Ca2+, Mg2+, and Li+ | Cellulophaga lytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulophaga lytica | A0A2I4KDZ0 | - |
- |
| Cellulophaga lytica N5-2 | A0A2I4KDZ0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Cellulophaga lytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | - |
Cellulophaga lytica | kappa-neocarraoctaose + kappa-neocarrahexaose | - |
? | |
| kappa-carrageen + H2O | - |
Cellulophaga lytica N5-2 | kappa-neocarraoctaose + kappa-neocarrahexaose | - |
? | |
| additional information | no activity with iota- or lambda-carrageenan, or any other polysaccharides such as agar, alginate, cellulose and starch | Cellulophaga lytica | ? | - |
? | |
| additional information | no activity with iota- or lambda-carrageenan, or any other polysaccharides such as agar, alginate, cellulose and starch | Cellulophaga lytica N5-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Cellulophaga lytica |
| ? | x * 55240, calculated from amino acid sequence | Cellulophaga lytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| kappa-car | - |
Cellulophaga lytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cellulophaga lytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | about 75% activity at 25°C, about 85% activity at 30°C, 100% activity at 35°C, about 95% activity at 40°C, about 90% activity at 45°C, about 55% activity at 50°C | Cellulophaga lytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
the enzyme retains more than 75% of its initial activity after heat treatment below 35°C for 2 h | Cellulophaga lytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cellulophaga lytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | about 25% activity at pH 4.0, about 47% activity at pH 5.0, about 75% activity at pH 6.0, 100% activity at pH 7.0, about 65% activity at pH 8.0, about 30% activity at pH 9.0 | Cellulophaga lytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 50% of the enzyme activity is maintained after incubation at pH 5.0-8.0 and 4°C for 6 h | Cellulophaga lytica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cellulophaga lytica | calculated from amino acid sequence | - |
9.9 |
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