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Literature summary for 3.2.1.83 extracted from

  • Sun, Y.; Liu, Y.; Jiang, K.; Wang, C.; Wang, Z.; Huang, L.
    Electrospray ionization mass spectrometric analysis of ?-carrageenan oligosaccharides obtained by degradation with kappa-carrageenase from Pedobacter hainanensis (2014), J. Agric. Food Chem., 62, 2398-2405.
    View publication on PubMed
Search for more literature for 3.2.1.83

Application

Application Comment Organism
additional information the enzymatic degradation offers an alternative approach to prepare kappa-carrageenan oligosaccharides, which could be used as a powerful tool for further study on biological activity-structure relationship and thorough industrial exploitation of kappacarrageenan Pedobacter hainanensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Pedobacter hainanensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kappa-carrageen + H2O Pedobacter hainanensis
-
 ?
-
 ?
kappa-carrageen + H2O Pedobacter hainanensis 13-Q
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pedobacter hainanensis
-
 isolated from seaside soil under the stacks of red algae in Hainan province of China
-
Pedobacter hainanensis 13-Q
-
 isolated from seaside soil under the stacks of red algae in Hainan province of China
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture supernatant by ammonium sulfate fractionation and adsorption chromatography Pedobacter hainanensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
700.5
-
 purified enzyme, pH 7.0, 40°C Pedobacter hainanensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageen + H2O
-
 Pedobacter hainanensis ?
-
 ?
kappa-carrageen + H2O
-
 Pedobacter hainanensis 13-Q ?
-
 ?
kappa-carrageen + H2O the enzyme cleaves kappa-carrageenan at the internal beta-1,4 linkage of kappa-carrageenan Pedobacter hainanensis carrageenan oligosaccharide the enzyme degrades kappa-carrageenan to sulfated oligosaccharides with even-numbered degree of polymerization, of which the tetrasaccharide was the major product, identification and structural analysis by mass spectrometry, overview. Each carrageenan oligosaccharide is composed of An-G4S-type neocarrabiose units, which consisted of a 3,6-anhydro-alpha-D-galactose residue in the nonreducing end and a beta-D-galactose-4-sulfate residue in the reducing end ?
kappa-carrageen + H2O the enzyme cleaves kappa-carrageenan at the internal beta-1,4 linkage of kappa-carrageenan Pedobacter hainanensis 13-Q carrageenan oligosaccharide the enzyme degrades kappa-carrageenan to sulfated oligosaccharides with even-numbered degree of polymerization, of which the tetrasaccharide was the major product, identification and structural analysis by mass spectrometry, overview. Each carrageenan oligosaccharide is composed of An-G4S-type neocarrabiose units, which consisted of a 3,6-anhydro-alpha-D-galactose residue in the nonreducing end and a beta-D-galactose-4-sulfate residue in the reducing end ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Pedobacter hainanensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pedobacter hainanensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pedobacter hainanensis