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Literature summary for 3.2.1.83 extracted from
- Characterization of a kappa-carrageenase from marine Cellulophaga lytica strain N5-2 and analysis of its degradation products (2013), Int. J. Mol. Sci., 14, 24592-24602.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km value for kappa-carrageenan is 1.647 mg/ml at 35°C and pH 7.0 | Cellulophaga lytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40800 | - |
x * 40800, SDS-PAGE | Cellulophaga lytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulophaga lytica | - |
isolated from the sediment of carrageenan production base in Hainan (Hainan, China) | - |
| Cellulophaga lytica N5-2 | - |
isolated from the sediment of carrageenan production base in Hainan (Hainan, China) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme to electrophoretic homogeneity from culture supernatant by ammonium sulfate fractionation, dialysis, and two different steps of gel filtration | Cellulophaga lytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1170 | - |
purified enzyme, pH 7.0, 35°C | Cellulophaga lytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | the enzyme is specific for the beta-1,4 linkage and hydrolyzes kappa-carrageenan into kappa-neocarraoctaose-sulfate and kappa-neocarrahexaose-sulfate first, and then brakes kappa-neocarraoctaose-sulfate into kappa-neocarrabiose-sulfate and kappa-neocarrahexaose-sulfate, NMR spectroscopy analysis, overview | Cellulophaga lytica | kappa-neocarrahexaose-sulfate + kappa-neocarrabiose-sulfate + kappa-neocarrahexaose-sulfate | - |
? |  |
| kappa-carrageen + H2O | the enzyme is specific for the beta-1,4 linkage and hydrolyzes kappa-carrageenan into kappa-neocarraoctaose-sulfate and kappa-neocarrahexaose-sulfate first, and then brakes kappa-neocarraoctaose-sulfate into kappa-neocarrabiose-sulfate and kappa-neocarrahexaose-sulfate, NMR spectroscopy analysis, overview | Cellulophaga lytica N5-2 | kappa-neocarrahexaose-sulfate + kappa-neocarrabiose-sulfate + kappa-neocarrahexaose-sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40800, SDS-PAGE | Cellulophaga lytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cellulophaga lytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range | Cellulophaga lytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, pH 7.0, 2.5 h, stable at | Cellulophaga lytica |
| 50 | - |
purified enzyme, pH 7.0, inactivation within 90 min | Cellulophaga lytica |
| 60 | - |
purified enzyme, pH 7.0, inactivation within 60 min | Cellulophaga lytica |
| 70 | - |
purified enzyme, pH 7.0, inactivation within 30 min | Cellulophaga lytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cellulophaga lytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | activity range | Cellulophaga lytica |
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Release 2023.1 (January 2023)
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