Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Zobellia sp. M-2 | |
Triton X-100 | - |
Zobellia sp. M-2 | |
Tween 80 | - |
Zobellia sp. M-2 |
Cloned (Comment) | Organism |
---|---|
gene cgkZ, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme with and without signal peptide extracellulary and intracellularly in Escherichia coli strain BL21 (DE3), cloning in Escherichia coli strain DH5alpha | Zobellia sp. M-2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Zobellia sp. M-2 | |
Cu2+ | - |
Zobellia sp. M-2 | |
EDTA | - |
Zobellia sp. M-2 | |
Mn2+ | - |
Zobellia sp. M-2 | |
Pb2+ | - |
Zobellia sp. M-2 | |
SDS | - |
Zobellia sp. M-2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
kappa-carrageen | pH 6.0, 39°C, recombinant enzyme | Zobellia sp. M-2 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme contains a signal peptide | Zobellia sp. M-2 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | poor effects by Li+, Mg2+, NH4+, Ca2+ and K+ at 5 mM | Zobellia sp. M-2 | |
Na+ | activates, best at 100 mM | Zobellia sp. M-2 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37300 | - |
x * 37300, recombinant His-tagged extracellular and intracellular enzyme, SDS-PAGE | Zobellia sp. M-2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zobellia sp. M-2 | the enzyme specifically cleaves the internal beta (1-4) linkages of carrageenans and yields a series of homologous even-numbered oligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zobellia sp. M-2 | S5U3X4 | i.e. strain ZM-2, gene cgkZ | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular (24fold) and intracellular (19fold) His-tagged enzyme from Escherichia coli strain BL21 (DE3) cell-free extract and culture supernatant by ammonium sulfate fractionation, dialysis, nickel affinity chromatography and dialysis | Zobellia sp. M-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Zobellia sp. M-2 | ? | - |
? | |
additional information | the enzyme specifically cleaves the internal beta (1-4) linkages of carrageenans and yields a series of homologous even-numbered oligosaccharides | Zobellia sp. M-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37300, recombinant His-tagged extracellular and intracellular enzyme, SDS-PAGE | Zobellia sp. M-2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Zobellia sp. M-2 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | high activity within this range | Zobellia sp. M-2 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified recombinant enzyme, pH 6.0, 3 h, 95% activity remaining | Zobellia sp. M-2 |
40 | - |
purified recombinant enzyme, pH 6.0, 1 h, 50% activity remaining | Zobellia sp. M-2 |
45 | - |
purified recombinant enzyme, pH 6.0, 15 min, 20% activity remaining | Zobellia sp. M-2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Zobellia sp. M-2 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | activity range | Zobellia sp. M-2 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
purified recombinant enzyme, 20°C, 2 h, 65% activity remaining | Zobellia sp. M-2 |
6 | 8 | purified recombinant enzyme, 20°C, 2 h, 85% activity remaining | Zobellia sp. M-2 |
9 | - |
purified recombinant enzyme, 20°C, 2 h, 65% activity remaining | Zobellia sp. M-2 |
10 | - |
purified recombinant enzyme, 20°C, 2 h, inactivation | Zobellia sp. M-2 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 16, GH16 | Zobellia sp. M-2 |