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Literature summary for 3.2.1.83 extracted from
- Enzymatic degradation of kappa-carrageenan in aqueous solution (2009), Biomacromolecules, 10, 1757-1767.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged enzyme expression in the periplasm of Escherichia coli strain BL21(DE3) | Pseudoalteromonas carrageenovora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaI | strong inhibition at 200 mM, inhibition is caused by iodide binding | Pseudoalteromonas carrageenovora |  |
| Tris | strong inhibition at 200 mM | Pseudoalteromonas carrageenovora | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageenan + H2O | Pseudoalteromonas carrageenovora | degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas carrageenovora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from the periplasm of Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography | Pseudoalteromonas carrageenovora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageenan + H2O | degradation | Pseudoalteromonas carrageenovora | ? | - |
? | |
| kappa-carrageenan + H2O | degradation, analysis of the degradation products by gel filtration, single-helix ordered conformation state for kappa-carrageenan. Degradation of carrageenan in the disordered state leads to a rapid decrease in molecular mass and the production of all possible neo-kappa-carrabiose oligomers | Pseudoalteromonas carrageenovora | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| kappa-carrageenase | - |
Pseudoalteromonas carrageenovora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Pseudoalteromonas carrageenovora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudoalteromonas carrageenovora |
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Release 2023.1 (January 2023)
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