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Literature summary for 3.2.1.82 extracted from
- Characterization of an exopolygalacturonase from Aspergillus niger (2008), Appl. Biochem. Biotechnol., 149, 205-217.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | properties of PGI may be suitable for food processing | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | at 1 mM causes 21% inhibition of PGI, at 5 mM causes 35% inhibition of PGI | Aspergillus niger |  |
| CoCl2 | at 1 mM causes 19% inhibition of PGI, at 5 mM causes 45% inhibition of PGI | Aspergillus niger | |
| CuSO4 | at 1 mM causes 27% inhibition of PGI, at 5 mM causes 48% inhibition of PGI | Aspergillus niger | |
| HgCl2 | at 1 mM causes 17% inhibition of PGI, at 5 mM causes 48% inhibition of PGI | Aspergillus niger | |
| MgCl2 | at 1 mM causes 8% inhibition of PGI, at 5 mM causes 22% inhibition of PGI | Aspergillus niger | |
| MnCl2 | at 1 mM causes 57% inhibition of PGI, at 5 mM causes complete inhibition of PGI | Aspergillus niger | |
| NiCl2 | at 1 mM causes 10% inhibition of PGI, at 5 mM causes 37% inhibition of PGI | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration of PGI | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger ATCC 9029 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PGI and PGII purified by gel filtration, PGI 12fold purified to homogeneity | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.7 | - |
cell-free broth of PGI | Aspergillus niger |
| 186.6 | - |
PGI and PGII | Aspergillus niger |
| 418.6 | - |
12fold purified PGI | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, PGI in liquid form, 2 weeks | Aspergillus niger |
| 4°C, PGI in powder form, 9 months | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | PGI activity on pectin decreases with an increase in degree of esterification. For pectins with different degrees of methylation, the extent of hydrolysis of PGI is increased with increasing of methoxyl content | Aspergillus niger | ? | - |
? | |
| pectin + H2O | PGI activity on pectin decreases with an increase in degree of esterification. For pectins with different degrees of methylation, the extent of hydrolysis of PGI is increased with increasing of methoxyl content | Aspergillus niger ATCC 9029 | ? | - |
? | |
| polygalacturonic acid + H2O | PGI | Aspergillus niger | ? | - |
? | |
| polygalacturonic acid + H2O | PGI | Aspergillus niger ATCC 9029 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE, PGI | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exopolygalacturonase | - |
Aspergillus niger |
| PGI | - |
Aspergillus niger |
| PGII | - |
Aspergillus niger |
| polygalacturonase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | PGI | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
PGI is stable up to 30°C | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53.4 | - |
Pectin | PGI, 8.9% degree of methylation of pectin | Aspergillus niger | |
| 85.4 | - |
polygalacturonic acid | PGI | Aspergillus niger | |
| 122.8 | - |
Pectin | PGI, 7.8% degree of methylation of pectin | Aspergillus niger | |
| 132.7 | - |
Pectin | PGI, 26% degree of esterification of pectin | Aspergillus niger | |
| 133.5 | - |
Pectin | PGI, 6% degree of methylation of pectin | Aspergillus niger | |
| 160.2 | - |
Pectin | PGI, 12% degree of methylation of pectin | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
PGI in sodium acetate buffer | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | PGI | Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectic focusing of PGI | - |
7.6 |
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