Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.4 mM Ca2+ shifts the optimal temperature from 50°C to 55°C, the activity is 1.2fold higher than the maximal activity without Ca2+ at 50°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
x * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q8RR74 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.1 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is unable to degrade citrus pectins with degrees of esterification of 63% and 93% | Bacillus sp. (in: Bacteria) | ? | - |
? | |
oligogalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | digalacturonate | - |
? | |
polygalacturonate + H2O | - |
Bacillus sp. (in: Bacteria) | digalacturonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
exo-PHase | - |
Bacillus sp. (in: Bacteria) |
exopolygalacturonase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
Tris-HCl buffer in presence of Ca2+ | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | 30°C: about 30% of maximal activity, 65°C: about 30% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 15 min, stable up to | Bacillus sp. (in: Bacteria) |
60 | - |
pH 7.0, 15 min, about 5% loss of activity | Bacillus sp. (in: Bacteria) |
65 | - |
pH 7.0, 15 min, about 90% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 55% of maximal activity, pH 10.0: about 35% of maximal activity | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | isoelectrofocusing, pH-gradient 3.5-9.5 | - |
5 |