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Literature summary for 3.2.1.82 extracted from
- Isolation and characterisation of an extracellular glycosylated protein complex from Clostridium thermosacchalyticum with pectin methylesterase and polygalacturonate hydrolase activity (1993), Appl. Environ. Microbiol., 59, 828-836.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
Pectin | - |
Thermoanaerobacterium thermosaccharolyticum |  |
| 0.08 | - |
pectate | - |
Thermoanaerobacterium thermosaccharolyticum | |
| 3.1 | - |
tetragalacturonate | - |
Thermoanaerobacterium thermosaccharolyticum | |
| 3.1 | - |
alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronic acid | - |
Thermoanaerobacterium thermosaccharolyticum | |
| 4.1 | - |
hexagalacturonate | - |
Thermoanaerobacterium thermosaccharolyticum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoanaerobacterium thermosaccharolyticum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Thermoanaerobacterium thermosaccharolyticum | - |
digalacturonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium thermosaccharolyticum | - |
protein complex with polygalacturonate hydrolase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermoanaerobacterium thermosaccharolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronic acid + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | ? | - |
? | |
| hexagalacturonate + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | ? | - |
? | |
| pectate + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | digalacturonate | - |
? | |
| pectin + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | digalacturonate | - |
? | |
| polygalacturonate + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | digalacturonate | - |
? | |
| tetragalacturonate + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pectate | Thermoanaerobacterium thermosaccharolyticum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | activity decreases rapidly at higher temperatures | Thermoanaerobacterium thermosaccharolyticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable for more than 1 h | Thermoanaerobacterium thermosaccharolyticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
pectate | Thermoanaerobacterium thermosaccharolyticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 7 | pectate | Thermoanaerobacterium thermosaccharolyticum |
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