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Literature summary for 3.2.1.81 extracted from

  • Li, R.K.; Chen, Z.; Ying, X.J.; Ng, T.B.; Ye, X.Y.
    A novel GH16 beta-agarase isolated from a marine bacterium, Microbulbifer sp. BN3 and its characterization and high-level expression in Pichia pastoris (2018), Int. J. Biol. Macromol., 119, 1164-1170 .
    View publication on PubMed
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Microbulbifer sp. BN3

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 89% residual activity at 2 mM Microbulbifer sp. BN3
EDTA 92% residual activity at 2 mM Microbulbifer sp. BN3
Fe2+ 77% residual activity at 2 mM Microbulbifer sp. BN3
Mn2+ 70% residual activity at 2 mM Microbulbifer sp. BN3
SDS 91% residual activity at 2 mM Microbulbifer sp. BN3
Zn2+ 81% residual activity at 2 mM Microbulbifer sp. BN3

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 115% activity at 2 mM Microbulbifer sp. BN3
Mg2+ 107% activity at 2 mM Microbulbifer sp. BN3
Na+ 112% activity at 2 mM Microbulbifer sp. BN3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agar + H2O Microbulbifer sp. BN3
-
 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?

Organism

Organism UniProt Comment Textmining
Microbulbifer sp. BN3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and HiTrap DEAE column chromatography Microbulbifer sp. BN3

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1237
-
 crude enzyme, at pH 6.0 and 50°C Microbulbifer sp. BN3
6447
-
 5.21fold purified enzyme, at pH 6.0 and 50°C Microbulbifer sp. BN3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Microbulbifer sp. BN3 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?
agarose + H2O
-
 Microbulbifer sp. BN3 neoagarobiose + neoagarotetraose
-
 ?
Gracilaria lemaneiformis + H2O
-
 Microbulbifer sp. BN3 neoagarobiose + neoagarotetraose
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Microbulbifer sp. BN3
? x * 34300, calculated from amino acid sequence Microbulbifer sp. BN3

Synonyms

Synonyms Comment Organism
N3-1 protein
-
 Microbulbifer sp. BN3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Microbulbifer sp. BN3

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 at 60 °C, the enzyme activity is 75% of the maximum activity, and enzyme activity declines sharply at 70°C Microbulbifer sp. BN3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is very stable after incubation for 30 min at 30-50°C, at 60°C 40% of the maximum enzyme activity is remaining Microbulbifer sp. BN3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Microbulbifer sp. BN3

pH Range

pH Minimum pH Maximum Comment Organism
4 8 within the range of pH 4.0 to 8.0, the enzyme shows over 60% activity Microbulbifer sp. BN3

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme is stable between pH 4.0-9.0 for 1 h. The enzyme retains over 70% activity after 30 min incubation at pH 6.0 and 50°C Microbulbifer sp. BN3

pI Value

Organism Comment pI Value Maximum pI Value
Microbulbifer sp. BN3 calculated from amino acid sequence
-
 5