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Literature summary for 3.2.1.81 extracted from
- Isolation of a novel freshwater agarolytic Cellvibrio sp. KY-YJ-3 and characterization of its extracellular beta-agarase (2010), J. Microbiol. Biotechnol., 20, 1378-1385.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Cellvibrio sp. |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cellvibrio sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Cellvibrio sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellvibrio sp. | - |
- |
- |
| Cellvibrio sp. KY-YJ-3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Toyopearl QAE-550C column chromatography, Toyopearl HW-55F column chromatography, and Mono-Q column chromatography | Cellvibrio sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.7 | - |
crude enzyme from culture fluid, in 50 mM phosphate buffer, pH 7.0, 35°C | Cellvibrio sp. |
| 84.2 | - |
after 120.2fold purification, in 50 mM phosphate buffer, pH 7.0, 35°C | Cellvibrio sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | specific substrate | Cellvibrio sp. | neoagarobiose + neoagarotetraose + neoagarohexaose | main products | ? |  |
| agarose + H2O | specific substrate | Cellvibrio sp. KY-YJ-3 | neoagarobiose + neoagarotetraose + neoagarohexaose | main products | ? | |
| additional information | the purified agarase fails to carboxymethyl-cellulose, dextran, soluble starch, pectin, and polygalacturonic acid | Cellvibrio sp. | ? | - |
? | |
| additional information | the purified agarase fails to carboxymethyl-cellulose, dextran, soluble starch, pectin, and polygalacturonic acid | Cellvibrio sp. KY-YJ-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Cellvibrio sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaA | - |
Cellvibrio sp. |
| beta-agarase | - |
Cellvibrio sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Cellvibrio sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | the agarase exhibits more than 90% of the maximum activity in the temperature ranging from 40°C to 50°C | Cellvibrio sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cellvibrio sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | the agarase exhibits more than 90% of the maximum activity in the pH range of 5.5 to 7.5 | Cellvibrio sp. |
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