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Literature summary for 3.2.1.81 extracted from

  • Jonnadula, R.; Ghadi, S.
    Purification and characterization of beta-agarase from seaweed decomposing bacterium Microbulbifer sp. strain CMC-5 (2011), Biotechnol. Bioprocess Eng., 16, 513-519.
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Activating Compound

Activating Compound Comment Organism Structure
NaCl 145% activity at 500 mM NaCl Microbulbifer sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 85% residual activity at 1 mM Microbulbifer sp.
Co2+ complete inhibition at 1 mM Microbulbifer sp.
Cu2+ complete inhibition at 1 mM Microbulbifer sp.
EDTA 46% residual activity at 1 mM Microbulbifer sp.
Fe2+ complete inhibition at 1 mM Microbulbifer sp.
Hg2+ complete inhibition at 1 mM Microbulbifer sp.
N-bromosuccinimide complete inhibition at 0.1 mM Microbulbifer sp.
NaCl 60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M Microbulbifer sp.
SDS complete inhibition at 1% (w/v) Microbulbifer sp.
Zn2+ complete inhibition at 1 mM Microbulbifer sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 123% activity at 1 mM Microbulbifer sp.
Mg2+ 112% activity at 1 mM Microbulbifer sp.
Mn2+ 105% activity at 1 mM Microbulbifer sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, SDS-PAGE Microbulbifer sp.

Organism

Organism UniProt Comment Textmining
Microbulbifer sp.
-
-
-
Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, DEAE Sepharose column chromatography and Sephacryl S-300HR gel filtration Microbulbifer sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 culture supernatant, pH 7.0, 30°C Microbulbifer sp.
4.22
-
 after 103.5fold purification, pH 7.0, 30°C Microbulbifer sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Microbulbifer sp. ?
-
 ?
agar + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
agarose + H2O
-
 Microbulbifer sp. neoagarotetraose + neoagarohexaose neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product ?
agarose + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) neoagarotetraose + neoagarohexaose neoagarotetraose and neoagarohexaose are the major products, neoagarooctaose is also observed as a minor end product ?
alginate + H2O
-
 Microbulbifer sp. ?
-
 ?
carboxymethyl cellulose + H2O
-
 Microbulbifer sp. ?
-
 ?
carboxymethyl cellulose + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
carrageenan + H2O
-
 Microbulbifer sp. ?
-
 ?
chitin + H2O
-
 Microbulbifer sp. ?
-
 ?
chitin + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?
xylan + H2O
-
 Microbulbifer sp. ?
-
 ?
xylan + H2O
-
 Microbulbifer sp. CMC-5 (MTCC 9889) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Microbulbifer sp.

Synonyms

Synonyms Comment Organism
endo-type beta-agarase
-
 Microbulbifer sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 96% of the agarase activity is still observed at 60°C Microbulbifer sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the agarase is thermally stable up to 50°C, with 62% of its residual activity is retained and 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C Microbulbifer sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Microbulbifer sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the agarase retains more than 70% of its original activity at pH 6.0 and 8.0 Microbulbifer sp.