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Literature summary for 3.2.1.81 extracted from

  • Oh, C.; Nikapitiya, C.; Lee, Y.; Whang, I.; Kim, S.J.; Kang, D.H.; Lee, J.
    Cloning, purification and biochemical characterization of beta agarase from the marine bacterium Pseudoalteromonas sp. AG4 (2010), J. Ind. Microbiol. Biotechnol., 37, 483-494.
    View publication on PubMed
Search for more literature for 3.2.1.81

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 2 mM Pseudoalteromonas sp. AG4
EDTA 2 mM inhibits the activity by 90% Pseudoalteromonas sp. AG4
Mn2+ 2 mM inhibits the activity by 40% Pseudoalteromonas sp. AG4
Zn2+ 2 mM inhibits the activity by 80% Pseudoalteromonas sp. AG4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM stimulates agarase activity by 5% Pseudoalteromonas sp. AG4
Fe2+ 2 mM stimulates agarase activity by 20% Pseudoalteromonas sp. AG4
K+ 2 mM stimulates agarase activity by 5% Pseudoalteromonas sp. AG4
Mg2+ 2 mM stimulates agarase activity by 5% Pseudoalteromonas sp. AG4
Na+ 2 mM stimulates agarase activity by 5% Pseudoalteromonas sp. AG4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 subunit, calculated from amino acid sequence Pseudoalteromonas sp. AG4
72500
-
 maltose binding protein-fused enzyme, SDS-PAGE Pseudoalteromonas sp. AG4

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. AG4 D7NPL0
-
-

Purification (Commentary)

Purification (Comment) Organism
using the pMAL protein fusion and purification system Pseudoalteromonas sp. AG4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
204.4
-
 using agar as substrate, in pH 5.5 acetate buffer, at 45°C Pseudoalteromonas sp. AG4
207.5
-
 using agarose as substrate, in pH 5.5 acetate buffer, at 45°C Pseudoalteromonas sp. AG4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Pseudoalteromonas sp. AG4 neoagarobiose + neoagarotetraose + neoagarohexaose
-
 ?
agarose + H2O
-
 Pseudoalteromonas sp. AG4 neoagarotetraose
-
 ?
additional information recombinant AgrP does not hydrolyze neoagarotetraose Pseudoalteromonas sp. AG4 ?
-
 ?
neoagarohexaose + H2O
-
 Pseudoalteromonas sp. AG4 neoagarobiose + neoagarotetraose
-
 ?

Synonyms

Synonyms Comment Organism
AgaP
-
 Pseudoalteromonas sp. AG4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudoalteromonas sp. AG4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme is stable below 55°C for 1 h Pseudoalteromonas sp. AG4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pseudoalteromonas sp. AG4

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 the enzyme retains its activity up to 60% at pH 4.5-7.5 Pseudoalteromonas sp. AG4

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8
-
 Pseudoalteromonas sp. AG4

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas sp. AG4 calculated from amino acid sequence
-
 5.9