Literature summary for 3.2.1.81 extracted from

Kim, H.T.; Lee, S.; Lee, D.; Kim, H.S.; Bang, W.G.; Kim, K.H.; Choi, I.G.
Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: an exo-type beta-agarase producing neoagarobiose (2010), Appl. Microbiol. Biotechnol., 86, 227-234.

Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharophagus degradans

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	3.2% residual activity at 10 mM	Saccharophagus degradans
Fe2+	50.5% residual activity at 10 mM	Saccharophagus degradans
K+	94.9% residual activity at 100 mM	Saccharophagus degradans
Mn2+	45% residual activity at 100 mM	Saccharophagus degradans
Zn2+	9.3% residual activity at 10 mM	Saccharophagus degradans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2	-	agarose	in 20 mM Tris-HCl buffer (pH 7.0), at 30°C	Saccharophagus degradans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	100 mM Ca2+ activates the enzyme activity 1.5times	Saccharophagus degradans
additional information	not affected by 10 mM Mn2+, Na+, K+, Ca+, and Mg2+	Saccharophagus degradans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
84000	-	His-tagged recombinant enzyme, SDS-PAGE	Saccharophagus degradans

Organism

Organism	UniProt	Comment	Textmining
Saccharophagus degradans	-	-	-
Saccharophagus degradans 2-40 / ATCC 43961	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Saccharophagus degradans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
agarose + H2O	Aga50D has unique exo-lytic activity and is able to produce neoagarobiose directly from agarose	Saccharophagus degradans	neoagarobiose	-	?
agarose + H2O	Aga50D has unique exo-lytic activity and is able to produce neoagarobiose directly from agarose	Saccharophagus degradans 2-40 / ATCC 43961	neoagarobiose	-	?

Synonyms

Synonyms	Comment	Organism
Aga50D	-	Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Saccharophagus degradans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	activity decreases sharply at temperatures higher than 40°C	Saccharophagus degradans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
22	-	agarose	in 20 mM Tris-HCl buffer (pH 7.0), at 30°C	Saccharophagus degradans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Saccharophagus degradans

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	more than 80% of the maximal activity retained	Saccharophagus degradans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.3	-	agarose	in 20 mM Tris-HCl buffer (pH 7.0), at 30°C	Saccharophagus degradans

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Release 2023.1 (January 2023)