Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 5 mM, complete inhibition of agarase AG-b | Vibrio sp. | |
EDTA | 5 mM, 15% inhibition of agarase AG-b | Vibrio sp. | |
Fe3+ | 1 mM, 65% inhibition of agarase AG-b | Vibrio sp. | |
Hg2+ | 5 mM, complete inhibition of agarase AG-b | Vibrio sp. | |
SDS | 5 mM, 16% inhibition of agarase AG-b | Vibrio sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, increases activity of agarase AG-b 1.2fold | Vibrio sp. | |
Cu2+ | 1 mM, increases activity of agarase AG-b 1.2fold | Vibrio sp. | |
Mn2+ | 1 mM, increases activity of agarase AG-b 1.4fold | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, agarase AG-a, SDS-PAGE | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
- |
- |
Vibrio sp. F-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
230.1 | - |
agarase AG-a | Vibrio sp. |
Storage Stability | Organism |
---|---|
4°C, pH 7.5 (phosphate buffer), 6 months, agarase AG-a loses 8% activity | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Vibrio sp. | neoagarotetraose + neoagarohexaose | main hydrolysates of agarose by agarase AG-a are neoagarotetraose (48%), neoagarohexaose (50%), and neoagarobiose (2%) | ? | |
agarose + H2O | - |
Vibrio sp. F-6 | neoagarotetraose + neoagarohexaose | main hydrolysates of agarose by agarase AG-a are neoagarotetraose (48%), neoagarohexaose (50%), and neoagarobiose (2%) | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, agarase AG-a, SDS-PAGE | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
agarase AG-a | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
agarase AG-a | Vibrio sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | 25°C: about 60% of maximal activity, 70°C: about 80% of maximal activity, agarase AG-a | Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, about 15% loss of activity, agarase AG-a | Vibrio sp. |
45 | - |
30 min, about 35% loss of activity, agarase AG-a | Vibrio sp. |
55 | - |
30 min, about 60% loss of activity, agarase AG-a | Vibrio sp. |
60 | - |
30 min, about 80% loss of activity, agarase AG-a | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
agarase AG-a | Vibrio sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 65% of maximal activity, pH 10.0: about 75% of maximal activity, agarase AG-a | Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 4°C, 6 h, stable in the pH range of 4.010.0, agarase AG-a | Vibrio sp. |