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Literature summary for 3.2.1.81 extracted from

  • Zhang, L.; Lu, X.; Han, F.; Ma, C.; Yu, W.
    Purification and refolding of a novel beta-agarase from inclusion body of E. coli (2007), J. Ocean Univ. China, 6, 80-84.
No PubMed abstract available
Search for more literature for 3.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
agaB gene inserted into vector pBV220, expressed in Escherichia coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag Pseudoalteromonas sp. CY24
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Escherichia coli
Cu2+
-
 Pseudoalteromonas sp. CY24
EDTA
-
 Escherichia coli
EDTA
-
 Pseudoalteromonas sp. CY24
Mn2+
-
 Escherichia coli
Mn2+
-
 Pseudoalteromonas sp. CY24

Localization

Localization Comment Organism GeneOntology No. Textmining
inclusion body
-
 Pseudoalteromonas sp. CY24 16234
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slightly increases activity Pseudoalteromonas sp. CY24
Ba2+ slightly increases the enzyme activity Escherichia coli
Ca2+ slightly increases activity Pseudoalteromonas sp. CY24
Ca2+ slightly increases the enzyme activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Escherichia coli
-
 neoagarooctaose + neoagarodecaose major products ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudoalteromonas sp. CY24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Escherichia coli
to electrophoretic purity by immobilized metal affinity chromatography Pseudoalteromonas sp. CY24

Renatured (Commentary)

Renatured (Comment) Organism
using 1 M urea, 500 mM L-arginine, 10% (w/v) glycerol and 20 mM sodium phosphate (pH 7.0), at 4°C Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1600
-
 purified refolded AgaB Pseudoalteromonas sp. CY24

Storage Stability

Storage Stability Organism
-20°C Pseudoalteromonas sp. CY24

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Pseudoalteromonas sp. CY24 neoagarooctaose + neoagarodecaose
-
 ?
agarose + H2O
-
 Escherichia coli neoagarooctaose + neoagarodecaose major products ?

Synonyms

Synonyms Comment Organism
AgaB
-
 Escherichia coli
agarase
-
 Escherichia coli
beta-agarase
-
 Pseudoalteromonas sp. CY24

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Escherichia coli
40
-
-
 Pseudoalteromonas sp. CY24

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Escherichia coli
6
-
-
 Pseudoalteromonas sp. CY24

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 10.6
-
 Pseudoalteromonas sp. CY24
5.7 10.6 the refolded enzyme is very stable when pH changed from 5.7 to 10.6 Escherichia coli