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Literature summary for 3.2.1.81 extracted from

  • Lee, D.G.; Park, G.T.; Kim, N.Y.; Lee, E.J.; Jang, M.K.; Shin, Y.G.; Park, G.S.; Kim, T.M.; Lee, J.H.; Lee, J.H.; Kim, S.J.; Lee, S.H.
    Cloning, expression, and characterization of a glycoside hydrolase family 50 beta-agarase from a marine Agarivorans isolate (2006), Biotechnol. Lett., 28, 1925-1932.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agarivorans sp.

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 optimal activity in presence of 1 mM NaCl and 1 mM CaCl2 Agarivorans sp.
NaCl optimal activity in presence of 1 mM NaCl and 1 mM CaCl2 Agarivorans sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.
109450
-
x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.

Organism

Organism UniProt Comment Textmining
Agarivorans sp.
-
-
-
Agarivorans sp. JA-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
167
-
pH 8.0, 40°C Agarivorans sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Agarivorans sp. neoagarosebiose main product ?
agarose + H2O
-
Agarivorans sp. JA-1 neoagarosebiose main product ?
neoagarohexaose + H2O
-
Agarivorans sp. neoagarobiose
-
?
neoagarohexaose + H2O
-
Agarivorans sp. JA-1 neoagarobiose
-
?
neoagarotetraose + H2O
-
Agarivorans sp. neoagarobiose
-
?
neoagarotetraose + H2O
-
Agarivorans sp. JA-1 neoagarobiose
-
?

Subunits

Subunits Comment Organism
? x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Agarivorans sp.
60
-
more than 70% of maximum activity Agarivorans sp.
70
-
more than 50% of maximum activity Agarivorans sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Agarivorans sp.