Literature summary for 3.2.1.81 extracted from

Ohta, Y.; Hatada, Y.; Nogi, Y.; Li, Z.; Zhang, H.; Ito, S.; Horikoshi, K.
Thermostable beta-agarase from a deep-sea Microbulbifer isolate (2004), J. Appl. Glycosci., 51, 203-210.

No PubMed abstract available

Search for more literature for 3.2.1.81

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	stabilizes enzyme	Microbulbifer sp.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Microbulbifer sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by EDTA, Na+, K+, Mg2+, Ca2+ and surfactants at high concentrations	Microbulbifer sp.
N-bromosuccinimide	enzyme protected of inhibition by agarose oligosaccharides	Microbulbifer sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	using Bacillus subtilis as the host	Microbulbifer sp.	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stabilizes enzyme	Microbulbifer sp.
K+	stabilizes enzyme	Microbulbifer sp.
Mg2+	stabilizes enzyme	Microbulbifer sp.
Na+	stabilizes enzyme	Microbulbifer sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65020	-	calculated from sequence	Microbulbifer sp.

Organism

Organism	UniProt	Comment	Textmining
Microbulbifer sp.	-	strain JAMB-A3	-
Microbulbifer sp. JAMB-A94	-	strain JAMB-A3	-

Purification (Commentary)

Purification (Comment)	Organism
-	Microbulbifer sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	isolated from the sediment in Sagami bay, Japan, at a depth of 1174m	Microbulbifer sp.	-

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
54	-	-	Microbulbifer sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	half-life of 8.7 h	Microbulbifer sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Microbulbifer sp.

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Release 2023.1 (January 2023)