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Literature summary for 3.2.1.81 extracted from

  • Ohta, Y.; Nogi, Y.; Miyazaki, M.; Li, Z.; Hatada, Y.; Ito, S.; Horikoshi, K.
    Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94 (2004), Biosci. Biotechnol. Biochem., 68, 1073-1081.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis ISW 1214 Microbulbifer sp.

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-(3-(3-dimethyl-amino-)propyl)carbonate 1 mM. 10-20% inhibition Microbulbifer sp.
Cu2+ 1 mM Microbulbifer sp.
diethyl dicarbonate 1 mM, 10-20% inhibition Microbulbifer sp.
Hg2+ 1 mM Microbulbifer sp.
additional information not inhibited by iodoacetamide, p-chloromercuribenzoate, dithiothreitol, 2-mercaptoethanol (each at 1 mM), EDTA (100 mM) and 30 mM SDS Microbulbifer sp.
N-bromosuccinimide 0.1 mM Microbulbifer sp.
Pb2+ 1 mM Microbulbifer sp.
Zn2+ 1 mM Microbulbifer sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
SDS-PAGE Microbulbifer sp.
48200
-
calculated from sequence Microbulbifer sp.

Organism

Organism UniProt Comment Textmining
Microbulbifer sp.
-
-
-
Microbulbifer sp. JAMB-A94
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification digestion during excretion Microbulbifer sp.

Purification (Commentary)

Purification (Comment) Organism
isolated from the sediment in Suruga bay, Japan, at a depth of 2406m, purification by ammonium sulfate precipitation, DEAE-Toyopearl 650M, gel filtration Microbulbifer sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information extracellular, using Bacillus subtilis as the host Microbulbifer sp.
-
additional information isolated from the sediment in Suruga bay, Japan, at a depth of 2406m Microbulbifer sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
517.3
-
-
Microbulbifer sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
Microbulbifer sp. neoagarotetraose + neoagarohexaose + neoagarobiose
-
?
agarose + H2O
-
Microbulbifer sp. JAMB-A94 neoagarotetraose + neoagarohexaose + neoagarobiose
-
?

Synonyms

Synonyms Comment Organism
AgaA
-
Microbulbifer sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
15 min in 20 mM Tris-HCl buffer, pH 7.0 Microbulbifer sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
retains 80% of original activity after 25 min Microbulbifer sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
Britton-Robinson universal buffers, 50°C Microbulbifer sp.