Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis ISW 1214 | Microbulbifer sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-(3-(3-dimethyl-amino-)propyl)carbonate | 1 mM. 10-20% inhibition | Microbulbifer sp. | |
Cu2+ | 1 mM | Microbulbifer sp. | |
diethyl dicarbonate | 1 mM, 10-20% inhibition | Microbulbifer sp. | |
Hg2+ | 1 mM | Microbulbifer sp. | |
additional information | not inhibited by iodoacetamide, p-chloromercuribenzoate, dithiothreitol, 2-mercaptoethanol (each at 1 mM), EDTA (100 mM) and 30 mM SDS | Microbulbifer sp. | |
N-bromosuccinimide | 0.1 mM | Microbulbifer sp. | |
Pb2+ | 1 mM | Microbulbifer sp. | |
Zn2+ | 1 mM | Microbulbifer sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Microbulbifer sp. |
48200 | - |
calculated from sequence | Microbulbifer sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer sp. | - |
- |
- |
Microbulbifer sp. JAMB-A94 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | digestion during excretion | Microbulbifer sp. |
Purification (Comment) | Organism |
---|---|
isolated from the sediment in Suruga bay, Japan, at a depth of 2406m, purification by ammonium sulfate precipitation, DEAE-Toyopearl 650M, gel filtration | Microbulbifer sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | extracellular, using Bacillus subtilis as the host | Microbulbifer sp. | - |
additional information | isolated from the sediment in Suruga bay, Japan, at a depth of 2406m | Microbulbifer sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
517.3 | - |
- |
Microbulbifer sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Microbulbifer sp. | neoagarotetraose + neoagarohexaose + neoagarobiose | - |
? | |
agarose + H2O | - |
Microbulbifer sp. JAMB-A94 | neoagarotetraose + neoagarohexaose + neoagarobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AgaA | - |
Microbulbifer sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
15 min in 20 mM Tris-HCl buffer, pH 7.0 | Microbulbifer sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
retains 80% of original activity after 25 min | Microbulbifer sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Britton-Robinson universal buffers, 50°C | Microbulbifer sp. |